We narrowed to 2,457 results for: sumo

1. pSC-PreE2

   Plasmid

   #52264

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSC-IntE2

   Plasmid

   #52265

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Human, Synthetic)
   

   Use

   Tags

   GST and GyrA-intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-SENP8 (full length))

   Plasmid

   #16361

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP8 full length (SENP8 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   human SENP8 full length, see Depositor Comments

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pThuAsol

   Plasmid

   #171675

   Purpose

   pThuAsol expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   SUMO-TEV-ThuAsol
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-SENP1 (catalytic domain)

   Plasmid

   #16356

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP1 catalytic domain (SENP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 419 to 644 catalytic domain of human S…

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-SENP2 (catalytic domain)

   Plasmid

   #16357

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP2 catalytic domain (SENP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 365 to 590 catalytic domain of human S…

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSC-PreE2

   Plasmid

   #52264

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC-IntE2

   Plasmid

   #52265

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Human, Synthetic)
   

   Use

   Tags

   GST and GyrA-intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-SENP8 (full length))

   Plasmid

   #16361

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP8 full length (SENP8 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   human SENP8 full length, see Depositor Comments

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pThuAsol

    Plasmid

    #171675

    Purpose

    pThuAsol expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    SUMO-TEV-ThuAsol
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MERS-CoV Mpro strain HCoV-EMC 3CL protease domain

    Plasmid

    #228646

    Purpose

    Bacterial expression of the MERS-CoV Mpro domain for biochemical assay.

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    MERS-Cov Mpro domain
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. MERS-CoV Mpro strain HCoV-EMC 3CL protease domain

    Plasmid

    #228646

    Purpose

    Bacterial expression of the MERS-CoV Mpro domain for biochemical assay.

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    MERS-Cov Mpro domain
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. CTA2-mNG211

    Plasmid

    #141167

    Purpose

    Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    SUMO-CTA2-mNG211
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 GATA1 K137R

    Plasmid

    #85694

    Purpose

    Mammalian expression of mouse GATA1 with K137R mutation

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K137R (SUMO-1 attachment site)

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

15. 6xHis-TEV-SMO-1(GG)

    Plasmid

    #87054

    Purpose

    Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    smo-1 (smo-1 Nematode)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    The last aa was removed to render processed SMO-1…

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid

    #208037

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    Ubnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Expression

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CTA2-mNG211

    Plasmid

    #141167

    Purpose

    Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    SUMO-CTA2-mNG211
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 GATA1 K137R

    Plasmid

    #85694

    Purpose

    Mammalian expression of mouse GATA1 with K137R mutation

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K137R (SUMO-1 attachment site)

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

19. 6xHis-TEV-SMO-1(GG)

    Plasmid

    #87054

    Purpose

    Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    smo-1 (smo-1 Nematode)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    The last aa was removed to render processed SMO-1…

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid

    #208037

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    Ubnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Expression

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge