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pBS-EF1-IRES-NEO-Control

Plasmid

#80757

Purpose

Transposon Reporter

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

NeoR (neoR Synthetic)

Promoter

EF1-IRES from PB-EF1-IRES-NEO

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKCZ_WT

Plasmid

#82228

Purpose

Gateway Donor vector containing PRKCZ , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCZ (PRKCZ Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZBTB24_WT

Plasmid

#82887

Purpose

Gateway Donor vector containing ZBTB24, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKACB_WT

Plasmid

#82224

Purpose

Gateway Donor vector containing PRKACB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKACB (PRKACB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRecLV105 MEF2C S222A

Plasmid

#107239

Purpose

A lentiviral vector for expression in mammalian cells encoding MEF2C-S222A insert

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C S222A

Use

Retroviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.25_S3-4-5-6

Plasmid

#173956

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP6C_p.R264C

Plasmid

#81341

Purpose

Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP6C (PPP6C Human)

Use

Gateway entry vector

Mutation

R264C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.D176H

Plasmid

#82870

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

D176H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.G251F

Plasmid

#82862

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

G251F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.G56V

Plasmid

#82760

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

G56V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.G242W

Plasmid

#82763

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

G242W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.G251V

Plasmid

#82765

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

G251V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.L245F

Plasmid

#82751

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

L245F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAGEC2_WT

Plasmid

#81862

Purpose

Gateway Donor vector containing MAGEC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAGEC2 (MAGEC2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T2-Ste7-EE

Plasmid

#52681

Purpose

expression of constitutively active Ste7 kinase in bacteria

Depositor

Benjamin Turk

Article

Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

Insert

Ste7 (STE7 Budding Yeast)

Tags

GST and Myc

Expression

Bacterial

Mutation

S359E/T363E constitutively active

Promoter

tac

Available Since

April 30, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223_TNFRSF14_WT

Plasmid

#81850

Purpose

Gateway Donor vector containing TNFRSF14 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFRSF14 (TNFRSF14 Human)

Use

Gateway entry vector

Mutation

K17R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXQ1

Plasmid

#101628

Purpose

Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXQ1 (FOXQ1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiSF3B1

Plasmid

#202446

Purpose

Expression of a CRISPRi guide targeting SF3B1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

SF3B1 gRNA (SF3B1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgKCMF1

Plasmid

#202441

Purpose

Expression of a guide RNA targeting KCMF1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

KCMF1 gRNA (KCMF1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLgw ELK4-V5-EcoDam

Plasmid

#98607

Purpose

Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Lentiviral; Damid

Tags

V5 and Dam (DNA adenine methyltransferase)

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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