We narrowed to 21,081 results for: Vars;

1. LentiU6-hASCL1 gRNA_1-MS2-Puro

   Plasmid

   #192670

   Purpose

   Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human ASCL1 activating gRNA #1 (ASCL1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS2021

   Plasmid

   #49128

   Purpose

   AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple302-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   DCX

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJC5-4-insulator-BREreporter-insulator

   Plasmid

   #171499

   Purpose

   injections to make Bmp reporter mouse transgenic line

   Depositor

   James Briscoe

   Article

   Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

   Insert

   Insulator BRE-MLP:H2B-Turqoise
   

   Expression

   Mammalian

   Promoter

   BRE-MLP

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

4. pET-KIX (586-672)

   Plasmid

   #99336

   Purpose

   Bacterial expression of mouse CBP KIX domain

   Depositor

   Peter Wright

   Article

   Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

   Insert

   CBP KIX (586-672) (Crebbp Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-PSAT

   Plasmid

   #74115

   Purpose

   Expression of PSAT

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   pET28a-PSAT (PSAT1 Human)
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KIF5C(1-560)-2xmCitrine-GFP11

   Plasmid

   #61667

   Purpose

   Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
   

   Expression

   Mammalian

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-LYRa11-RBD-8h

   Plasmid

   #161822

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T324-D522)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRC/CMV-JAK1K908E-VSV

   Plasmid

   #139357

   Purpose

   Expression of kinase-dead JAK1-K908E protein

   Depositor

   Sandra Pellegrini

   Article

   Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

   Insert

   JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   K908E

   Promoter

   CMV

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-RsSHC014-RBD-8h

   Plasmid

   #161826

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-SARS-1-RBD-8h

    Plasmid

    #161821

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLX306 iCre tetO NICD rtTA

    Plasmid

    #158049

    Purpose

    lenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domain

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    mouse Notch Intra Cellular Domain
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    tetO promoter

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pETM10 with rat FL Annexin A11

    Plasmid

    #164496

    Purpose

    For bacterial expression of rat His-tagged FL Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat FL Annexin A11 (Anxa11 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR2.1_HBB_N

    Plasmid

    #87847

    Purpose

    pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

    Depositor

    Marina Kleanthous

    Article

    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

    Insert

    Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid

    #169737

    Purpose

    Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEMS1997

    Plasmid

    #49127

    Purpose

    AAV plasmid with SLC6A4 (Ple198) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple198-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    SLC6A4

    Available Since

    May 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGAPDH::H2B-mScarlet

    Plasmid

    #186764

    Purpose

    A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    H2B-mScarlet
    

    Use

    Overexpression

    Tags

    H2B

    Promoter

    GAPDH

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-hIP-dCas9-BSD

    Plasmid

    #183231

    Purpose

    Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    Human insulin promoter

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPKm-248

    Plasmid

    #90504

    Purpose

    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    

    Use

    Lentiviral

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2143

    Plasmid

    #104356

    Purpose

    AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-smCBA-EmGFP-WPRE
    

    Use

    AAV

    Promoter

    smCBA

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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