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pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX-he1.1:YFP

Plasmid

#184816

Purpose

Used to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDR274-gsc2-sgRNA

Plasmid

#184818

Purpose

Used to synthesize gRNA targeting exon 2 of the gsc2 gene

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

gsc2-sgRNA

Use

CRISPR; Template for sgrna synthesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-nmbb

Plasmid

#184822

Purpose

Used to synthesize RNA probe for in situ hybridization for nmbb

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

nmbb (nmbb Zebrafish)

Use

Template for synthesis of nmbb probe

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-nts

Plasmid

#184823

Purpose

Used to synthesize RNA probe for in situ hybridization for nts

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

nts (nts Zebrafish)

Use

Template for synthesis of nts probe

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-mApple-he1.1:CFP

Plasmid

#184814

Purpose

Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-mApple-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-mApple

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-GFP-he1.1:CFP

Plasmid

#184815

Purpose

Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-GFP-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-GFP

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 125-175

Plasmid

#99668

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain (125-175)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-RTA(75-190)

Plasmid

#99669

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

VP64-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (1-100)

Plasmid

#99660

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (1-100)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (50-150)

Plasmid

#99661

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (50-150)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-Rta Full (1-190)

Plasmid

#99662

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain (1-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 75-190

Plasmid

#99663

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain (75-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 75-175

Plasmid

#99666

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain (75-175)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (150-261)

Plasmid

#99655

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (150-261)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (100-261)

Plasmid

#99656

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (100-261)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (200-261)

Plasmid

#99657

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (200-261)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (1-200)

Plasmid

#99658

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (1-200)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (1-150)

Plasmid

#99659

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated p65 activation domain (1-150)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL682

Plasmid

#205591

Purpose

PE2 for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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