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PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Mutation

See depositor comments below.

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(F74G)-V5

Plasmid

#158664

Purpose

Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(F74G) AAVS1

Use

CRISPR

Expression

Mammalian

Mutation

F74G

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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WDR5-HaloTag

Plasmid

#200881

Purpose

Mammalian expression of human WDR5 with C-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

WDR5-HaloTag (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-M.SssI (dCas9-M.SssI)

Plasmid

#218782

Purpose

Expresses dCAS9-M.SssI fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-M.SssI (dCas9-M.SssI)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeonGreen-P2A-mCherry

Plasmid

#229600

Purpose

Expresses mNeonGreen and mCherry in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_APOBEC1-UdgX-nCas9

Plasmid

#163531

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-T2A-GFP-PGK-Puro

Plasmid

#203607

Purpose

Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pN3-sTRAIL

Plasmid

#154246

Purpose

Plasmid compatible with non-viral delivery for secretion of trimeric human TRAIL

Depositor

Jordan Green

Article

Vaughan et al Mol Ther Oncolytics. 2021 Apr 16;21:377-

Insert

Secretable Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand

Expression

Mammalian

Promoter

CMV Promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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