We narrowed to 18,398 results for: Mut

1. pMD2.G-H8A/K47Q

   Plasmid

   #207322

   Purpose

   VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H8A/K47Q

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Expression

   Mutation

   Promoter

   TgTUB1 and Toxoplasma U6

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Integrin-β6 Headpiece I270C

   Plasmid

   #159638

   Purpose

   Contains integrin beta 6 residues 1–474 with I270C mutation

   Depositor

   Timothy Springer

   Article

   Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

   Insert

   Integrin beta 6 headpiece I270C
   

   Use

   Tags

   6x His

   Expression

   Mammalian

   Mutation

   I270C

   Promoter

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA HA FAK K454R

   Plasmid

   #50501

   Depositor

   Kenneth Yamada

   Article

   Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K454R

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Integrin-αv Headpiece M400GC

   Plasmid

   #159637

   Purpose

   Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

   Depositor

   Timothy Springer

   Article

   Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

   Insert

   alpha v headpiece M400GC
   

   Use

   Tags

   6x His and Strep II

   Expression

   Mammalian

   Mutation

   M400C

   Promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB1-moxGFP

   Plasmid

   #103984

   Purpose

   Plasmid encoding moxGFP

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   moxGFP
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S30R, Y39N, …

   Promoter

   proC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAM1.16_PB-Cx43_S368A-PGK-Blast

   Plasmid

   #205561

   Purpose

   Express human Cx43 with S368A mutation

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Cx43
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   S368A

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET24b-Amg2

   Plasmid

   #199434

   Purpose

   expresses Amg2 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   Amg2
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   L159K mutation

   Promoter

   T7

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50472

    Purpose

    Gs-coupled rM3D-IRES-mCitrine under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    rM3D-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5'Entry-lck-7.4kb

    Plasmid

    #58891

    Purpose

    provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
    

    Use

    Gateway 5' entry clone

    Tags

    Expression

    Mutation

    mutated single nucleotide (T-->C, 1874th nucle…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pReporter_3

    Plasmid

    #60564

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pReporter_4

    Plasmid

    #60565

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. AICSDP-39:RAB5A-mEGFP

    Plasmid

    #107579

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5A

    Depositor

    Allen Institute for Cell Science

    Insert

    RAB5A Homology Arms with mEGFP-linker (RAB5A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    May 9, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEB1-mVenus ME

    Plasmid

    #103989

    Purpose

    Plasmid encoding mVenus ME

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus ME
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAC_N_STA5A

    Plasmid

    #167799

    Purpose

    Expresses STA5A in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    STA5A (STAT5A Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pALS3-Ma-sfGFP-TAG150

    Plasmid

    #212121

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Kv-ArcLight-ST

    Plasmid

    #182319

    Purpose

    Soma-targeted fluorescent voltage indicator with left-shifted voltage-dependency

    Depositor

    Rafael Yuste

    Article

    Bando et al Nat Commun. 2021 Dec 10;12(1):7229. doi:

    Insert

    Kv-ArcLight-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation in an amino acid Q217E of ArcLight-MT

    Promoter

    CAG

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLG029 (mzaABCDE)

    Plasmid

    #157907

    Purpose

    Expresses the mzaABCDE defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    mzaABCDE (5-gene system containing a MutL homolog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50470

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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