We narrowed to 16,515 results for: grna

Showing: 11061 - 11080 of 16515 results

Results per page:

Sort results by:

pRRL-mU6-GFPGO2-IRES-mScarletI

Plasmid

#136896

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2-IRES-mScarletI

Use

Lentiviral

Tags

2xNLS on GFP, 3xNLS on mScarlet

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasSA

Plasmid

#98211

Purpose

CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

Depositor

Quanjiang Ji

Article

Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Aurora A

Plasmid

#198402

Purpose

Expression of EGFP-tagged Aurora A in mammalian cells

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Aurora A

Tags

EGFP

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA-gRic6T

Plasmid

#106401

Purpose

E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

Depositor

Sheng Yang

Article

Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

Insert

sgRNA

Expression

Bacterial

Promoter

pj23119

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2-scr

Plasmid

#169795

Purpose

Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

Scrambled guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p8092 LentiCRISPR v2 sgNT-1

Plasmid

#163315

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgNT-1

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAf-CRISPR-yA

Plasmid

#191015

Purpose

CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

Depositor

Perng-Kuang Chang

Article

Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

Insert

yA

Use

CRISPR

Promoter

Aspergillus flavus U6

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ TREX1-KI

Plasmid

#127701

Purpose

For knocking in TREX1 in mouse - Doxycyclin inducible

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

mouse TREX1 cDNA (Trex1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas9-sgAAVS1-2

Plasmid

#129727

Purpose

Expressing SpCas9 and sgAAVS1-2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-2

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-shNTC

Plasmid

#217638

Purpose

Dox inducible shRNA non targeting control shRNA

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shNTC

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CROPseq-Puro-4xMS2

Plasmid

#212626

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-STOP-shA1

Plasmid

#14422

Depositor

Klaus Rajewsky

Article

Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-90

Insert

modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pk335-BFP

Plasmid

#227505

Purpose

Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAS9i

Plasmid

#141249

Purpose

Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

Depositor

Adam Bertl

Article

Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

GAL1 (Cas9), pSNR52 (gRNA)

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PTEN shRNA

Plasmid

#86645

Purpose

expression of shRNA targeting PTEN

Depositor

Jaime Modiano

Article

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (PTEN Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge