We narrowed to 16,198 results for: grna

1. pBK1290-AAV-dSaCas9

   Plasmid

   #223139

   Purpose

   AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MLSE-shREN

   Plasmid

   #105583

   Purpose

   retrovirally express control shRNA with GFP marker

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   control shRNA targeting luciferase
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   MSCV-LTR

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnxABE-flgn

   Plasmid

   #187932

   Purpose

   A-to-G base editor

   Depositor

   Yi Xiao

   Article

   et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

   Insert

   ecTadA(8e)-nxCas9 (D10A)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-puro-sh-mFAK C

   Plasmid

   #37015

   Depositor

   Bob Weinberg

   Article

   Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

   Insert

   sh-mFAK C (Ptk2 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 31, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

   Plasmid

   #124861

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. THI VP64 pALD4 T-

   Plasmid

   #161481

   Purpose

   Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Non targeting control NTC)
   

   Expression

   Yeast

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. BII-Sh-PnGW

   Plasmid

   #133359

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   GFP-NLS
   

   Tags

   HA-tagged GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSicoR human DGCR8-1

   Plasmid

   #14769

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   DGCR8 shRNA (DGCR8 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pXPR_212

   Plasmid

   #133457

   Purpose

   U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTX1-SMN1-exon7-ESE1

    Plasmid

    #126041

    Purpose

    In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    SMN1 exon7 ESE1
    

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_213

    Plasmid

    #133456

    Purpose

    H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    Control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMP-Suv39H1_1

    Plasmid

    #36342

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Suv39H1 (SUV39H1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pBrain-GFP-shTACC3

    Plasmid

    #59355

    Purpose

    Knocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    EGFP
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUPER.ATRi

    Plasmid

    #14582

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    ATR shRNA (ATR Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

15. TRMPV-Neo

    Plasmid

    #27990

    Purpose

    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

    Depositor

    Scott Lowe

    Article

    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

    Insert

    TRMPV-Neo (shRen)
    

    Use

    RNAi and Retroviral; Fluorescent reporters

    Expression

    Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRMPV-Hygro

    Plasmid

    #27996

    Purpose

    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

    Depositor

    Scott Lowe

    Article

    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

    Insert

    TRMPV-Hygro (shLuci-1309)
    

    Use

    RNAi and Retroviral; Fluorescent reporters

    Expression

    Mammalian

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBO.ACT005

    Plasmid

    #182715

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSuperRetropuro-IL-6 shRNA 1

    Plasmid

    #19959

    Depositor

    Christopher Counter

    Article

    Ancrile et al Genes Dev. 2007 Jul 15. 21(14):1714-9.

    Insert

    pSuperRetropuro-IL-6 shRNA 1 (IL6 Human)
    

    Use

    RNAi and Retroviral

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pEn-C1.1

    Plasmid

    #61479

    Purpose

    Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

    Depositor

    Holger Puchta

    Article

    Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUB-Cas9-@GL1

    Plasmid

    #86779

    Purpose

    Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    sgRNA against GLABROUS1
    

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    Arabidopsis U6-26 promoter

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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