168,004 results

1. pcDNA3puro-mGFP-ROCK2

   Plasmid

   #225472

   Purpose

   Express mEGFP-fusion protein of ROCK2

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   ROCK2 (ROCK2 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2908

   Plasmid

   #143663

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFIC (NFIC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Tags

   GFP11x7

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. RFP Dynamin2 K44A

   Plasmid

   #128153

   Purpose

   Expression of Dynamin 2 K44A

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

   Insert

   Dynamin 2 (Dnm2 Rat)
   

   Expression

   Mammalian

   Mutation

   K44A

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRANS_250d

   Plasmid

   #91124

   Purpose

   Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   PvUbi2

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Kif5A-GFP-CIBN

   Plasmid

   #102252

   Purpose

   Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   Kif5A-GFP-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-HA-ER Y537S

   Plasmid

   #49499

   Purpose

   Expresses HA-tagged ER Y537S mutant in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tyrosine 537 to Serine

   Promoter

   CMV

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-VAMP8

   Plasmid

   #45912

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   vesicle-associated membrane protein 8 (VAMP8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

9. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL20_mEGFP_KMT2A_MLLT1

    Plasmid

    #205842

    Purpose

    Express mEGFP-tagged fusion protein, KMT2A_MLLT1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    KMT2A_MLLT1 (KMT2A, MLLT1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcdna nesprin TS

    Plasmid

    #68127

    Purpose

    nesprin tension sensor

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    mouse nesprin mini 2G (Syne2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX551

    Plasmid

    #60957

    Purpose

    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV1)

    Viral Prep

    #83898-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    mDlx

    Tags

    mCherry

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

    Plasmid

    #55209

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    10xAsn, 10xHis, MPB, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. RAD21 C-tag CRISPR

    Plasmid

    #140540

    Purpose

    RAD21 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNR220

    Plasmid

    #79541

    Purpose

    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    TP901
    BxbI
    A118
    

    Tags

    His tag and LAA degradation tag

    Expression

    Bacterial

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAPK8

    Plasmid

    #73236

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK8 (MAPK8 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 26-321

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid

    #183831

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    L1196M/G1202R

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SP1-SB100X

    Plasmid

    #154887

    Purpose

    Sleeping Beauty transposase fused to SP1

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    SP1-Sleeping Beauty
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-L1PA1CH blast

    Plasmid

    #69612

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Tags

    blast cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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