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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

Plasmid

#107729

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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RPS6KA1 gRNA (BRDN0001148103)

Plasmid

#75498

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA1 (RPS6KA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-1.1-2.01 beta

Plasmid

#69591

Purpose

OT-II TCR beta chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR beta clone 1.1 (Tcrb Mouse)

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pEMS1265

Plasmid

#29137

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple223 (TBR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB3

Plasmid

#19471

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB3 (Dnajb3 Mouse)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_PGE2-1.0-IRES-mCherry-CAAX

Plasmid

#251353

Purpose

Express the PGE2 sensor GRAB_PGE2-1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Neuron. 2026 Jun 3;114(11):1922-1934.e5.

Insert

PGE2 sensor GRAB_PGE2-1.0

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-Lyzc-mCherry-Rab4 DN

Plasmid

#254774

Purpose

Neutrophil specific Dominant Negative Rab4 in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rab4 (RAB4A Human)

Use

Zebrafish expression

Mutation

DN(S27N)

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(sgReg1-1)-PGKpuro2ABFP-W

Plasmid

#252911

Purpose

Expression vector for gRNA with Puro and tagBFP selection markers

Depositor

Sok Ching Cheong

Insert

ZC3H12A (ZC3H12A Human)

Use

CRISPR

Promoter

human U6 promoter, mouse PGK promoter

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(sgReg1-2)-PGKpuro2ABFP-W

Plasmid

#252912

Purpose

Expression vector for gRNA with Puro and tagBFP selection markers

Depositor

Sok Ching Cheong

Insert

ZC3H12A (ZC3H12A Human)

Use

CRISPR

Promoter

human U6 promoter, mouse PGK promoter

Available Since

April 28, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD02-EcHailibu

Plasmid

#254559

Purpose

For the expression of the Hailibu system encoded by Escherichia coli NCTC11131

Depositor

Philip Kranzusch

Insert

EcDUF4297, EcHerA

Expression

Bacterial

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD02-EcHachiman

Plasmid

#254558

Purpose

For the expression of the Hachiman system encoded by Escherichia coli ECOR31 NZ_QOXQ01000009

Depositor

Philip Kranzusch

Insert

EcHamA, EcHamB

Expression

Bacterial

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Mpp7-PDZ-1

Plasmid

#250109

Purpose

Bacterial expression of Mpp7 PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Mpp7 (1/1) (Mpp7 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Pdzk11-PDZ-1

Plasmid

#250128

Purpose

Bacterial expression of Pdzk11 PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Pdzk11 (1/1) (Pdzd11 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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TRAV1-1

Plasmid

#237951

Purpose

Encodes TRAV1-1 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV1-1 (TRAV1-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BQ.1.1)

Plasmid

#241111

Purpose

Expresses SARS-CoV-2 spike (BQ.1.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BQ.1.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,H69del,V70del,G142…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_2xRRM_pGEX_Flag_noSBP

Plasmid

#228796

Purpose

Recombinant expression of mouse MSI1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 (Msi1 Mouse)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV26-1

Plasmid

#237983

Purpose

Encodes TRAV26-1 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV26-1 (TRAV26-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV13-1

Plasmid

#237970

Purpose

Encodes TRAV13-1 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV13-1 (TRAV13-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV38-1

Plasmid

#237991

Purpose

Encodes TRAV38-1 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV38-1 (TRAV38-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV12-1

Plasmid

#237967

Purpose

Encodes TRAV12-1 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV12-1 (TRAV12-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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