We narrowed to 27,562 results for: sta

1. pCas12a

   Plasmid

   #186446

   Purpose

   Encodes Cas12a from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SadCas9 KRAB

   Plasmid

   #188504

   Purpose

   Expresses FLAG tagged SadCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGCP123-GFP_g1

   Plasmid

   #153518

   Purpose

   sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. G-GECO1-Orai3

   Plasmid

   #73563

   Purpose

   Green fluorescent reporter of Orai3-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1-Orai3 (ORAI3 Synthetic, Human)
   

   Use

   Tags

   G-GECO1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immdeiate Early

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Dest47- C11orf83-GFP

   Plasmid

   #65844

   Purpose

   Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   UQCC3 WT (UQCC3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5 mSynCAM 2

   Plasmid

   #115497

   Purpose

   expression of mouse full-length SynCAM 2

   Depositor

   Thomas Biederer

   Article

   Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

   Insert

   Cadm2 (Cadm2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. human EB1 in PET28a for protein expression

   Plasmid

   #39297

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Article

   Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCAG2IG-Hmga2

   Plasmid

   #122294

   Purpose

   Overexpression for mouse Hmga2

   Depositor

   Yukiko Gotoh

   Article

   Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

   Insert

   Hmga2 (Hmga2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. wt dynamin 1 pEGFP

   Plasmid

   #34680

   Depositor

   Sandra Schmid

   Article

   Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

   Insert

   dynamin 1ba (DNM1L Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCEP4-BG505.SOSIP-gp160

    Plasmid

    #123273

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV_JAK3_IRES_BFP

    Plasmid

    #206291

    Purpose

    Expresses the wild type form of human JAK3.

    Depositor

    Charles de Bock

    Article

    Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

    Insert

    JAK3 (JAK3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP HDAC5 WT

    Plasmid

    #32211

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pRedCm

    Plasmid

    #176225

    Purpose

    LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB and PL

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. His_Naa80d in pTYB12

    Plasmid

    #188455

    Purpose

    Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    Naa80 (NAA80 Human)
    

    Use

    Tags

    intein-CBD, 6xHis

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-delta90GFP

    Plasmid

    #26645

    Depositor

    Anjen Chenn

    Article

    Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

    Insert

    delta90 beta-catenin (Ctnnb1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deletion of first 90 aa of beta catenin

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-E2F-1 wt-pRcCMV

    Plasmid

    #21667

    Depositor

    William Kaelin

    Article

    Krek et al Cell. 1994 Jul 15. 78(1):161-72.

    Insert

    E2F-1 (E2F1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type-

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-MBP-CBX1-eReader

    Plasmid

    #135089

    Purpose

    Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-U6a-U6c-ndufs2 guide

    Plasmid

    #107601

    Purpose

    An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    ndufs2 guide RNA sequences
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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