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pcDNA3.1 NKX6-2-Venus T252A

Plasmid

#203717

Purpose

human NKX6-2 T252A phosphoresistant mutant

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (T252A) fused with Venus (NKX6-2 Human)

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (S252A)

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus T252D

Plasmid

#203718

Purpose

human NKX6-2 T252D phosphomimic mutant

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (T252D) fused with Venus (NKX6-2 Human)

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (S252D)

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-S13S2DBD

Plasmid

#200196

Purpose

Expression of Sox13 with Sox2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-S13S2DBD (Sox2, Sox13 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

Sox13(1-1242); Sox 2 (126-333); Sox13 (1449-1839)

Promoter

EF_1alpha

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ control-FLAG

Plasmid

#199678

Purpose

Bacterial expression of Leucine Zipper (LZ) control-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper control

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-S2S13DBD

Plasmid

#200195

Purpose

Expression of Sox2 with Sox13 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-S2S13DBD (Sox2, Sox13 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

Sox2(1-126); Sox13 (1242-1449); Sox2 (333-957)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

Plasmid

#196877

Purpose

Neuron-specific expression of mOrange2 reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-td-mCherry-CI

Plasmid

#196851

Purpose

Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-F1103A-dsRNAres_Y

Plasmid

#148044

Purpose

Insect Expression of DmPAN2-F1103A-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-F1103A-dsRNAres (PAN2 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y

Plasmid

#148064

Purpose

Insect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del130-133G-T97RH102G-dsRNAres (PAN2 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del246-249-dsRNAres_Y

Plasmid

#148065

Purpose

Insect Expression of DmPAN2-del246-249-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del246-249-dsRNAres (PAN2 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y

Plasmid

#148033

Purpose

Insect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del130-133G-T97RH102G-dsRNAres (PAN2 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-del246-249-dsRNAres_Y

Plasmid

#148034

Purpose

Insect Expression of DmPAN2-del246-249-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del246-249-dsRNAres (PAN2 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-del358-467-T97RH102G-dsRNAres_Y

Plasmid

#148036

Purpose

Insect Expression of DmPAN2-del358-467-T97RH102G-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del358-467-T97RH102G-dsRNAres (PAN2 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmTral_1-84_C

Plasmid

#146082

Purpose

Bacterial Expression of DmTral_1-84

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_1-84 (tral Fly)

Expression

Bacterial

Mutation

one silent mutation A198C compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNanos-del50-236_AC

Plasmid

#148459

Purpose

Insect Expression of DmNanos-del50-236

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-del50-236 (nos Fly)

Expression

Insect

Mutation

sequence contains a deletion of N283 and a mutati…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1710-2505-V2002E-dsRNAres_AC

Plasmid

#148463

Purpose

Insect Expression of DmNot1_1710-2505-V200E-dsRNAres

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNot1_1710-2505-V200E-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1710-2505-H2067D-dsRNAres_AD

Plasmid

#148500

Purpose

Insect Expression of DmNot1_1710-2505-H2067D-dsRNAres

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNot1_1710-2505-H2067D-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot3_607-748-Y702A-NpM-HsNot2_350-540_AB

Plasmid

#148334

Purpose

Bacterial Expression of HsNot3_607-748-Y702A-HsNot2_350-540

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

HsNot3_607-748-Y702A-HsNot2_350-540 (CNOT3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1

Plasmid

#192470

Purpose

Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS8

Tags

AcGFP1

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

Plasmid

#192469

Purpose

Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

AcGFP1

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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