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pCI-ING1b

Plasmid

#79052

Purpose

expression of p33ING1b in mammalian cells

Depositor

Karl Riabowol

Article

Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

Insert

ING1 (p33 ING1Ab isoform) (ING1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLV-75-2

Plasmid

#73036

Purpose

Express an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cells

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Insert

p75-2

Use

Lentiviral

Expression

Mammalian

Mutation

Mature Human NT-3 gene exchanged Aspartic 15 to a…

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67295

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-retroCHMP3-HA

Plasmid

#154178

Purpose

expresses mouse retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKmyc-Borg3

Plasmid

#141074

Purpose

Expresses Borg3 (Cdc42ep5) in mammalian cells

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

Insert

Borg3 (Cdc42ep5 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-SECFP

Plasmid

#175838

Purpose

Express SECFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with nucleus-targeting sequence

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP1_rpoA-U

Plasmid

#190975

Purpose

Test the U-to-C editing activity of KP1 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP1

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-MGT1-d2EGFP_CMV-mCherry

Plasmid

#213763

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

MGT#1-d2EGFP

Use

Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAP G116

Plasmid

#123099

Purpose

Expresses 3xFLAG-GABARAP G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-TO-HA-SH3BP1 (RNAi-res)

Plasmid

#136645

Purpose

Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (RNAi resistant) (SH3BP1 Human)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS4

Plasmid

#218713

Purpose

Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS4 (BBS4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

I354T (t1110c)

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAFMW-AthHEN1

Plasmid

#104980

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_744

Plasmid

#176663

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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3'-3xFLAG minimal CMV pCDNA5

Plasmid

#212045

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Myc-SNAP29

Plasmid

#116944

Purpose

Expressing Myc-SNAP29 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-empty

Plasmid

#221239

Purpose

Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-4xFKBP scaffold

Use

Retroviral

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE

Plasmid

#67292

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Myt1L (Myt1l Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-LbCas12a

Plasmid

#213743

Purpose

For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJZC583

Plasmid

#62314

Purpose

sgRNA with 2x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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BirA*-HA-NES

Plasmid

#214473

Purpose

Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

Depositor

Natalie Ahn

Article

Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

Insert

BirA(R118G)

Use

Lentiviral; Gateway

Tags

HA tag and NES (Nuclear Exclusion Sequence)

Expression

Mammalian

Mutation

R118G

Promoter

EF-1a

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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