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HA-VHL 1-167-pRc/CMV

Plasmid

#22078

Depositor

William Kaelin

Publication

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

deletion mutant covering only aa 1-167 of wt VHL …

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG-IRES2-mCherry

Plasmid

#175507

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Publication

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PB-CT3G-CERP2-MG-IRES2-mTagBFP2

Plasmid

#175509

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Publication

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSB1C3 I0500 LuxI

Plasmid

#107965

Purpose

Arabinose-inducible 3OC6HSL sender plasmid

Depositor

Jim Haseloff

Publication

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

luxI

Use

Synthetic Biology

Expression

Bacterial

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCoofy16

Plasmid

#43988

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10-NusA

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223_PYGL_WT_V5

Plasmid

#82991

Purpose

Gateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PYGL (PYGL Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DOK1_WT

Plasmid

#82884

Purpose

Gateway Donor vector containing DOK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DOK1 (DOK1 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEpic

Plasmid

#84372

Purpose

3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Lentiviral

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPINMSYB

Plasmid

#41118

Depositor

Ray Owens

Publication

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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All-in-one lenti-BE4-NG

Plasmid

#200448

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Publication

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pNP1 sfGFP trigger

Plasmid

#107359

Purpose

Trigger sequence for pNP1 sfGFP toehold switch

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 7

Expression

Bacterial

Promoter

T7

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_SHC1_WT_V5

Plasmid

#82931

Purpose

Gateway Donor vector containing SHC1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SHC1 (SHC1 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRF-LitR

Plasmid

#49363

Depositor

Christopher Voigt

Publication

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

LitR repressor

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRF-AmtR

Plasmid

#49355

Depositor

Christopher Voigt

Publication

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

AmtR repressor

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLT06

Plasmid

#255019

Purpose

Markerless deletion vector that enables manipulation of enterococcus to either insert DNA or delete DNA from the genome. Contains resistance gene for chloramphenicol resistance.

Depositor

Lynn Hancock

Publication

Thurlow et al J Bacteriol. 2009 Oct;191(20):6203-10. d

Type

Empty backbone

Available since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR223_GEN1_WT

Plasmid

#81844

Purpose

Gateway Donor vector containing GEN1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GEN1 (GEN1 Human)

Use

Gateway Cloning

Mutation

S92T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.G333C

Plasmid

#82835

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway Cloning

Mutation

G333C; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 CreERT2 L3-L2

Plasmid

#62174

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Publication

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

CreERT2

Use

Gateway Cloning

Expression

Mammalian

Promoter

SV40

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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PEW-pEF1A-inactEGFP

Plasmid

#212009

Purpose

Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

Depositor

Catherine Wu

Publication

Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

Insert

Inactivated eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

pEF1a

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HT110_FCMV_QuasAr6b_citrine

Plasmid

#178823

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Publication

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6b_citrine

Use

Lentiviral

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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