We narrowed to 4,746 results for: MLS

1. pETDuet-solo-NHis6-NocO-E94A

   Plasmid

   #240401

   Purpose

   expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

   Depositor

   Emily Balskus

   Article

   Wang et al Biochemistry. 2025 May 20;64(10):2173-21

   Insert

   nocO
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M1 of native sequence not included in insert, Glu…

   Promoter

   T7

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG391

   Plasmid

   #226732

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG369

   Plasmid

   #226748

   Purpose

   CRISPRi guide RNA against EMC2

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   EMC2 (EMC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG426

   Plasmid

   #226734

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   RHOT1 (RHOT1 Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG517

   Plasmid

   #226735

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG43

   Plasmid

   #226736

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MICU1 (targeting sequence) (MICU1 Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. H2B-GFP-INCENP∆cen

   Plasmid

   #184044

   Purpose

   Expresses H2B-GFP fused to INCENP∆cen

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   INCENP (INCENP Human)
   

   Use

   Tags

   GFP and H2B

   Expression

   Mammalian

   Mutation

   Truncated to 1-862

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG NC

   Plasmid

   #214675

   Purpose

   Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags

   Depositor

   Eugene Yeo

   Article

   Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   FLAG
   

   Use

   Tags

   MCP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pROS13-Tps2/Gsy1

   Plasmid

   #196613

   Purpose

   Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   S000001911 (TPS2, GSY1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-uTEV1-MAVS-mTA3

    Plasmid

    #171001

    Purpose

    HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIV]

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

    Plasmid

    #159664

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

    Plasmid

    #159663

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

    Plasmid

    #159662

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

    Plasmid

    #159656

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-EGxxFP-PIGA

    Plasmid

    #153958

    Purpose

    Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    PIGA (a 401-bp fragment from intron 5 and exon 6)
    

    Use

    A plasmid specific for egxxfp assay

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    No mutation

    Promoter

    CAG promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Donor-PIGAmut

    Plasmid

    #153548

    Purpose

    Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
    

    Use

    Donor plasmid

    Tags

    N/A

    Expression

    Mutation

    a 3-bp mutation in exon 6

    Promoter

    No promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

    Plasmid

    #81239

    Purpose

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAL4DBD-SuUR f.l

    Plasmid

    #112995

    Purpose

    P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

    Insert

    SuUR (SuUR Fly)
    

    Use

    P insertion vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. [pSK480] pLJC5 Flag-Depdc5(A)

    Plasmid

    #109338

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant A

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    DIYGD(185-189)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCerulean3-Keratin-17

    Plasmid

    #55424

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only