We narrowed to 1,604 results for: N-pac

1. pCAG-SpCas9-P2A-EGFP (MSP2582)

   Plasmid

   #223067

   Purpose

   pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized SpCas9-P2A-EGFP
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188743

   Purpose

   Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   RSV

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188744

   Purpose

   Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188745

   Purpose

   Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   nonTATAchr21

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188746

   Purpose

   Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   nonTATAchrX

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT244 Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast

   Plasmid

   #195537

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NES-mCherry-PH-BTKx1

   Plasmid

   #183653

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Frog, Human)
   

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

   Plasmid

   #172597

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-K627R (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-K627R

   Promoter

   CMV

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

   Plasmid

   #172596

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-R914W (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-R914W

   Promoter

   CMV

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter

    Plasmid

    #172598

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-D842V (PDGFRA Synthetic, Human)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-D842V

    Promoter

    CMV

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter

    Plasmid

    #172599

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-D842V+R914W

    Promoter

    CMV

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter

    Plasmid

    #172600

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-K627R+R914W

    Promoter

    CMV

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUGW-H1 empty vector

    Plasmid

    #25870

    Purpose

    3rd generation lentiviral vector

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1_3XFlag

    Plasmid

    #182494

    Purpose

    Empty plasmid containing Flag tag for C-terminal tagging

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

    Insert

    3xFlag with 5 Glycolic spacer
    

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC_sgRNA

    Plasmid

    #68710

    Purpose

    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Available Since

    Sept. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCO706

    Plasmid

    #139743

    Purpose

    Individual component of Caffeic Acid biosynthesis pathway, hpaC

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    hpaB
    

    Use

    Moclo level 0 component

    Promoter

    none

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCO708

    Plasmid

    #139745

    Purpose

    Individual component of Caffeic Acid biosynthesis pathway, hpaC

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    TAL
    

    Use

    Moclo level 0 component

    Promoter

    none

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDS_TempRecording

    Plasmid

    #241789

    Purpose

    pBBR1MCS replicative vector for Cas1-Cas2-RT and Spacer for transcriptional recording with a temperature sensor

    Depositor

    Marc Güell

    Article

    Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

    Insert

    FBFP, Cas1-Cas2-RT_and CRISPR Array
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSmart-EF1a-Nla-cas11

    Plasmid

    #178882

    Purpose

    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas11c-bGH polyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSmart-EF1a-Nla-cas8

    Plasmid

    #178880

    Purpose

    Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas8c-bGH PolyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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