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We narrowed to 14,232 results for: crispr grnas

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Showing: 1101 - 1120 of 14232 results

  3. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available Since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    70763-plasmid-map-sequence-id-240922

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  4. p425_Cas9_gRNA_LEU_1014a

    Plasmid
    #87407
    Purpose
    p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87407-plasmid-map-sequence-id-193449

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  5. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  7. pYJ18-PB-U6-Mafa-acti-gRNA1

    Plasmid
    #131062
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA1 (MAFA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131062-plasmid-map-sequence-id-256743

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  8. pYJ19-PB-U6-Mafa-acti-gRNA2

    Plasmid
    #131063
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA2 (MAFA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131063-plasmid-map-sequence-id-256773

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  9. pYJ20-PB-U6-Mafa-acti-gRNA3

    Plasmid
    #131064
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA3 (MAFA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131064-plasmid-map-sequence-id-256740

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  13. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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  15. pLenti-hRAB23-gRNA3

    Plasmid
    #249236
    Purpose
    RAB23 CRISPR KO
    Depositor
    Weihong Tan
    Insert
    RAB23 (RAB23 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    Feb. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    249236-plasmid-map-sequence-id-499789

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  16. pLenti-hRAB23-gRNA2

    Plasmid
    #249235
    Purpose
    RAB23 CRISPR KO
    Depositor
    Weihong Tan
    Insert
    RAB23 (RAB23 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    Feb. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    249235-plasmid-map-sequence-id-499788

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  17. pLenti-hRAB23-gRNA1

    Plasmid
    #249234
    Purpose
    RAB23 CRISPR KO
    Depositor
    Weihong Tan
    Insert
    RAB23 (RAB23 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    Feb. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    249234-plasmid-map-sequence-id-499786

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Showing: 1101 - 1120 of 14232 results