We narrowed to 13,434 results for: crispr grnas

1. pGH224_sgRNA_2xMS2_Puro

   Plasmid

   #85413

   Purpose

   hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. M-NM-sgRNA

   Plasmid

   #48673

   Purpose

   Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. p184_LTJ_sgRNACD90.2

   Plasmid

   #82670

   Purpose

   sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting mouse CD90.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. M-SP-sgRNA

   Plasmid

   #48671

   Purpose

   Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. XX>sgRNA F+E

   Plasmid

   #87543

   Purpose

   Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

   Depositor

   Lionel Christiaen

   Article

   Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p183_LTJ_sgRNACD90.2_A

   Plasmid

   #82671

   Purpose

   sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting CD90.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EC0_6_sgRNA

   Plasmid

   #163717

   Purpose

   Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   sgRNA expression cassette
   

   Use

   Synthetic Biology; Moclo vector level 0 (type 234)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p240_LTJ_sgRNAFoxp3K276X

   Plasmid

   #82675

   Purpose

   sgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting Foxp3 K276X
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p204_LTJ_sgRNACD45.2_R2

   Plasmid

   #82673

   Purpose

   sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting CD45.2 region 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p163_LTJ_sgRNACD90.1

    Plasmid

    #82677

    Purpose

    sgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD90.1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p206_LTJ_sgRNACD45.2_R3

    Plasmid

    #82674

    Purpose

    sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

    Plasmid

    #196846

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

    Plasmid

    #196847

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop

    Plasmid

    #196844

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

    Plasmid

    #196845

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

    Plasmid

    #120293

    Purpose

    AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    N-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40-NLS and split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p426-SNR52p-gRNA.csr-1.Y-SUP4t

    Plasmid

    #68060

    Purpose

    gRNA for csr-1 locus in N. crassa

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for csr-1 locus
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC-CR7T-gRNA2.1-nlsBFP

    Plasmid

    #170515

    Purpose

    gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CR7T

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgRNA1_Tet-inducible Luciferase reporter

    Plasmid

    #64161

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for Tet-inducible Luciferase reporter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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