We narrowed to 25,057 results for: Nes

1. pUC118-FLIP-FlpE-Puro

   Plasmid

   #84539

   Purpose

   a mock vector for generating FLIP-FlpE targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP-FlpE - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. RCAN1

   Plasmid

   #65413

   Purpose

   Full length RCAN1 expression with CMV promoter. Flag tagged.

   Depositor

   Jeffery Molkentin

   Article

   Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

   Insert

   RCAN1 (Rcan1 Mouse)
   

   Use

   Tags

   Flag tagged

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS79 (eft-3p::Cas9 + sgRNA)

   Plasmid

   #154839

   Purpose

   Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SETDB1

   Plasmid

   #25240

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1, SETDB1_16 (SETDB1 Human)
   

   Use

   Tags

   6xHis-TEV and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pDY1461_SERPINA1_CCA32_5avidity Cre

   Plasmid

   #193193

   Purpose

   Express Cre conditional upon RNA transcript

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC150-TEX264-GFP

   Plasmid

   #201931

   Purpose

   PiggyBac Vector expressing TEX264-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS415_pGal-nCas9(D10A)

   Plasmid

   #79618

   Purpose

   Expresses nCas9(D10A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVTH-sip53

   Plasmid

   #12239

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Insert

   shRNA against p53 (TP53 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMA-SpCas9-g2

   Plasmid

   #80785

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E_unc503

    Plasmid

    #64020

    Purpose

    5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    503-bp promoter of zebrafish unc-45b

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-FKBP-SAC1

    Plasmid

    #108127

    Purpose

    Recruitable SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRU319

    Plasmid

    #167686

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PBMBP-eGFP

    Plasmid

    #40976

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Insert

    MBP promoter fragment (Mbp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-AMOT p130 Y242/287A

    Plasmid

    #32822

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

    Insert

    AMOT p130 (AMOT Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E5D, Y242/287A

    Promoter

    CMV

    Available Since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pHACK-GAL4>QF2(newHA1)

    Plasmid

    #104873

    Purpose

    HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    T2A-QF2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

    Plasmid

    #49772

    Purpose

    Level 2 construct

    Depositor

    Sophien Kamoun

    Article

    Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

    Insert

    sgRNA_PDS2-Cas9-sgRNA_PDS1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-sav

    Plasmid

    #32834

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    Sav (SAV1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

19. FL-mCherry

    Plasmid

    #78862

    Purpose

    DULIP negative control (prey) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-TRIM28deltaRBCC

    Plasmid

    #124953

    Purpose

    Mammalian expression of Human TRIM28 with deleted RING, Bbox, CC domains

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RING, Bbox, and CC domains

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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