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Plasmid#138364PurposeLentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selectionDepositorInsertnuclear EGFP
UseLentiviralExpressionMammalianAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
myc-KIF5C
Plasmid#127618PurposeN-terminal myc-tagged mouse KIF5CDepositorAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-NR2F2
Plasmid#138359PurposeGateway compatible middle entry clone contains human full length NFR2F2 gene with stop codonDepositorInsertHuman NR2F2
ExpressionMammalianAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-C-CreintG (AAV1)
Viral prep#69571-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-C-CreintG (#69571). In addition to the viral particles, you will also receive purified pAAV-EF1a-C-CreintG plasmid DNA. EF1a-driven expression of optimized C-terminal component of Cre recombinase dependent on GFP (Cre-DOG OPT); to be coinjected with pAAV-EF1a-N-CretrcintG (Addgene catalog# 69570) and GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-N-CretrcintG (AAV1)
Viral prep#69570-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-N-CretrcintG (#69570). In addition to the viral particles, you will also receive purified pAAV-EF1a-N-CretrcintG plasmid DNA. EF1a-driven expression of optimized N-terminal component of Cre recombinase dependent on GFP (Cre-DOG OPT); to be coinjected with pAAV-EF1a-C-CreintG (Addgene catalog# 69571) and GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1
Plasmid#131725PurposeMammalian Expression of RAF1DepositorAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Cas9-NRRH
Plasmid#136924PurposeExpresses Cas9-NRRH in mammalian cellsDepositorInsertCas9-NRRH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSNG-P2A-Puro
Plasmid#136900PurposeLentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)DepositorInsertFNLSNG
UseLentiviralTagsFLAG-NLS and NLSMutationL1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337RPromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAF123
Plasmid#135921PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseTransposonExpressionMammalianAvailable sinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYH11-NanoLuc-2A-tdTomato
Plasmid#132569PurposeDonor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 alleleDepositorInsertHuman MYH11 knockin homology arms (MYH11 Human)
ExpressionMammalianAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC2A5
Plasmid#132090PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A5 (SLC2A5 Human)
ExpressionMammalianAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pD21-AI-hLDLR
Plasmid#124964PurposeExpress human LDLR under human APOAI promoterDepositorAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:GW
Plasmid#132358PurposeExpression of CDS from the Zea mays ubiquitin promotorDepositorTypeEmpty backboneTagsYFPExpressionPlantPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A12
Plasmid#131938PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A12 (SLC25A12 Human)
ExpressionMammalianAvailable sinceOct. 17, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)
Plasmid#132544PurposeExpresses CIRTS-6 (bdefensin 3-TBP6.7-YTHDF2(1-200)) in mammalian cellsDepositorInsertCIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)
TagsFLAG tagExpressionMammalianPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH-A3A-PBE
Plasmid#119774PurposeConversion of C to T in rice plants transformationDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAB10 T23N
Plasmid#130885PurposeExpresses human RAB10 T23N mutant with N-terminal GFP tagDepositorAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
(SUMO)10
Plasmid#126948PurposeBacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
ExpressionBacterialPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)3
Plasmid#127093PurposeBacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
TagsEGFPExpressionBacterialPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-imp α
Plasmid#119718PurposeExpresses EGFP-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
TagsEGFPExpressionMammalianMutationcontains amino acids 251-529PromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only