We narrowed to 13,397 results for: icos

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AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB450

Plasmid

#74832

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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PDGFR-TMD (MTK 3B) (pZYW025)

Plasmid

#194218

Purpose

Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PDGFR TMD

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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T2A-mCherry (MTK 4a) (pAN3844)

Plasmid

#194223

Purpose

Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

T2A-mCherry

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB507

Plasmid

#74849

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSEL1

Plasmid

#23011

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

DHFR (Dhfr Mouse)

Tags

6 His Tag (not in frame) and Xpress epitope (not …

Expression

Bacterial

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pGWB523

Plasmid

#74865

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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MSP2262

Plasmid

#70703

Purpose

Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

D10A & H557A in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB435

Plasmid

#68700

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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rat Kv3.1-HA

Plasmid

#113055

Purpose

Expresses rat Kv3.1 in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

rat Kv3.1 (Kcnc1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB423

Plasmid

#74817

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTHMT_hnRNPF_FL

Plasmid

#139114

Purpose

express FL hnRNPF with N-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Expression

Bacterial

Mutation

None

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLEG(R1-R4) #BGV057

Plasmid

#48957

Purpose

Lentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
PROM_MpUBE2

Plasmid

#136109

Purpose

PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_MpUBE2

Plasmid

#136110

Purpose

5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGI3EM09

Plasmid

#135482

Purpose

Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr hph-GFP ADH1t

Tags

GFP

Expression

Bacterial and Yeast

Promoter

SpunHsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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asPink chromoprotein promoter-less

Plasmid

#118005

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, asPink

Use

Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pVRc20_992

Plasmid

#49739

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS20_992

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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