We narrowed to 30,602 results for: JUN
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Plasmid#178051PurposeGFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.DepositorInsertToll-Interacting Protein (TOLLIP Human)
TagsGFPExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172670PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertSDHB pegRNA and SDHB_nick-sgRNA
ExpressionMammalianPromoterU6Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC299 VEGF Site#2
Plasmid#47506Purposehuman gRNA expression vector targeting VEGFDepositorAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry
Plasmid#83490Purposecre-dependent CA-AMPK under EF1a promoterDepositorInsertCA-AMPK-2A-mCherry
UseAAV and Cre/LoxExpressionMammalianMutationH150RAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT5T_hp53_ST
Plasmid#34922DepositorInsertp53 DNA-binding (Res 1-393) (TP53 Human)
ExpressionBacterialMutationC135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …PromoterT7Available SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133b
Plasmid#103223PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133b target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133b target (MIR133B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
VVT1
Plasmid#65779PurposeThe Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNADepositorInsertSaCas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-luciferase
Plasmid#113352Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseLuciferaseAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Actin-7
Plasmid#58001PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorAvailable SinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABE-puro SHP2 D61A
Plasmid#8330DepositorAvailable SinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98H-pRc/CMV
Plasmid#22044DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
TagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 98 from Tyrosine to H…Available SinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Cre
Plasmid#47117PurposeCan be used to in vitro transcribe Cre recombinase.DepositorInsertCre
PromoterLacAvailable SinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNAIIIB HA JNK
Plasmid#61630Purposemammalian expression of JNKDepositorAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGEX Tec-SH2
Plasmid#46522DepositorInsertTec (TEC Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 236-351PromoterTacAvailable SinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
ATP9A-Tol2
Plasmid#113385Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseTol2 reporterAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i4 sgRNA / hSpCas9
Plasmid#172828PurposeMammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP9A-luciferase
Plasmid#113354Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseLuciferaseAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Golgi-7
Plasmid#57959PurposeLocalization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565DepositorInsertGolgi (B4GALT1 Human)
TagsmOrange2ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-7-5p
Plasmid#103723PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-7-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only