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pCMV5B-Smad4

Plasmid

#11743

Depositor

Jeff Wrana

Article

Macias-Silva et al Cell. 1996 Dec 27. 87(7):1215-24.

Insert

SMAD4 (Smad4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pTalpha1-iCre

Plasmid

#133924

Purpose

Neuron-specific expression of iCre

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

iCre

Use

Tags

Expression

Mammalian

Mutation

Codon optimized

Promoter

neuron-specific tubulin-alpha-1 promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-SOCS1

Plasmid

#129514

Purpose

Retroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Article

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-q ONE-GO

Plasmid

#189738

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-q ONE-GO
GNAQ (Gnaq Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

Plasmid

#34877

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Peft-3 GFP H2B tbb-2 UTR

Use

Worm targeting

Tags

Expression

Mutation

S65C

Promoter

eef-1A.1 (eft-3)

Available since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pMT406

Plasmid

#139384

Purpose

RPU standard, pNBU1 backbone with BsaI sites, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

RPU standard

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

No promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8f-WPRE

Plasmid

#162376

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-ARP2-C-14

Plasmid

#53992

Purpose

Localization: Actin Related Protein, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

ARP2 (ACTR2 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Tags

Expression

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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