We narrowed to 15,824 results for: LEA

1. JDW 1045 (pLenti6.2_CMV_V5_TCF7L2_P2A_H2A_mCherry)

   Plasmid

   #233541

   Purpose

   A CMV driven human TCF7L2 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF7L2 (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCD-GSDMA-N-tev-C

   Plasmid

   #226231

   Purpose

   Express TEVP-cleavable GSDMA

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   Gasdermin A (Gsdma3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. piggyBAC-H2B mCherry-XL (R125K)

   Plasmid

   #189926

   Purpose

   Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

   Insert

   mCherry-XL (R125K)
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   differs from mCherry by R125K, W143S, I161V, Q163…

   Promoter

   CMV7

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Orai1 YFP

   Plasmid

   #19756

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTP1172

   Plasmid

   #104161

   Purpose

   Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL_U2AF1_WT_mCherry

   Plasmid

   #84017

   Purpose

   expression of U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. 14UAS PSD95:GFP 5UAS DSRedExpress

   Plasmid

   #74315

   Purpose

   Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

   Depositor

   Stephen Smith, Martin Meyer

   Article

   Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

   Inserts

   zebrafish PSD95
   DsRedExpress
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Y599H and I793S (please see depositor comment bel…

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGSKU

   Plasmid

   #72243

   Purpose

   Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

   Depositor

   Michael Resnick, Francesca Storici

   Article

   Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

   Inserts

   KlURA3
   kanMX4
   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6His-MBP-MpAgo

   Plasmid

   #79249

   Purpose

   This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

   Depositor

   Jennifer Doudna

   Article

   Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

   Insert

   Marinitoga piezophila Argonaute
   

   Use

   CRISPR

   Tags

   6XHis-tag + MBP + PreScission Cleavage Site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. WasCFP /pQE-30

    Plasmid

    #79858

    Purpose

    Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00

    Insert

    WasCFP
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Cyan fluorescent protein mCerulean with substitut…

    Promoter

    T5 promoter / lac operator

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. TTVTFLPTPSLILQT_epitope

    Plasmid

    #165021

    Purpose

    Expression of CMV UL44 (38-52) fused to CD74

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL44 (38-52)
    

    Use

    Lentiviral

    Tags

    CD74 at N-term; cathepsin S cleavage site (GRWHTV…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b PKA Cat

    Plasmid

    #14921

    Depositor

    Susan Taylor

    Article

    Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

    Insert

    PKA catalytic subunit alpha (Prkaca Mouse)
    

    Use

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL_U2AF1_S34F_mCherry

    Plasmid

    #84019

    Purpose

    expression of mutant U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    S34F

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCS mRFP

    Plasmid

    #91765

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

    Insert

    monomeric Red Fluorescent Protein1
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMNb-cALFA

    Plasmid

    #159991

    Purpose

    For the protein expression with ALFA tag at C-terminus in mammalian cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-twin Strep …

    Expression

    Mammalian

    Mutation

    CMV promoter, WPRE

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSC-NLS-EGFP

    Plasmid

    #221811

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NLS-EGFP
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRel-Hyg

    Plasmid

    #227582

    Purpose

    Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Venus-CAAX

    Plasmid

    #87612

    Purpose

    Plasma membrane-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFPC1-EPSIN 1

    Plasmid

    #22228

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    please see depositor comment below

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. AmCyan-P2A-mCherry

    Plasmid

    #45350

    Depositor

    Ilpo Huhtaniemi

    Article

    Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

    Insert

    AmCyan
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SV40 NLS

    Promoter

    CMV

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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