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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,245 results for: LEA

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Showing: 11341 - 11360 of 20245 results

  1. pHL 1477

    Plasmid
    #69787
    Purpose
    KanR with FRT sites + ptet::OmpC::T7RNAP::T1 terminator + ColE
    Depositor
    Han Lim
    Publication
    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw
    Insert
    T7RNAP
    Use
    Synthetic Biology
    Tags
    OmpC leader
    Expression
    Bacterial
    Promoter
    pTet
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. pST50Trc4-FLAGHISNDHFR

    Plasmid
    #64001
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64001-plasmid-map-sequence-id-177554

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  3. pST50Trc2-FLAGHISNDHFR

    Plasmid
    #63965
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63965-plasmid-map-sequence-id-177498

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  5. pZucchini2_SFFV-RARA-eGFP-IRES-mCherry_NeomycinR

    Plasmid
    #247925
    Purpose
    Overexpression vector for eGFP tagged RARA or NR1B1. Monitor post-translational degradation of RARA via EGFP:mCherry ratio. Contains neomycin-resistance selection marker.
    Depositor
    Benjamin Ebert, Jonathan Tsai
    Publication
    Tsai et al Mol Cell. 2023 Aug 3;83(15):2753-2767.e1
    Insert
    Retinoic Acid Receptor Alpha (RARA Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247925-plasmid-map-sequence-id-496527

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  6. pTol1-U6abc_erbb2_cmlc2-nmKate

    Plasmid
    #238409
    Purpose
    Drives expression of 3 different gRNAs targeting erbb2, and expression of nuclear mKate in cardiomyocytes
    Depositor
    Juan Manuel González-Rosa
    Publication
    Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuclear mKate/3 gRNAs targeting tnnt2a
    Promoter
    cmlc2 (nmKate); U6 (gRNAs)
    Available since
    Nov. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    238409-plasmid-map-sequence-id-472954

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  8. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  11. AP2u2-CMV-S_protein

    Plasmid
    #226456
    Purpose
    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Publication
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Insert
    Spike Protein
    Tags
    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…
    Expression
    Mammalian
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226456-plasmid-map-sequence-id-458388

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Showing: 11341 - 11360 of 20245 results