We narrowed to 15,981 results for: LEA

1. pGBW-m4046508

   Plasmid

   #145419

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4046357

   Plasmid

   #145417

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4046539

   Plasmid

   #145389

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGBW-m4046345

   Plasmid

   #145381

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4046283

   Plasmid

   #145383

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046546

   Plasmid

   #145384

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046507

   Plasmid

   #145385

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCI-neo-lambdaN-UPF1-DEAA

   Plasmid

   #136007

   Purpose

   DEAA UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Tags

   LambdaN

   Expression

   Mammalian

   Mutation

   DE647AA and S743A (Please see depositor comments …

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTK3_028

   Plasmid

   #124054

   Purpose

   Encodes c120 sg RNA guide cleaved by ribozyme

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   RIbozyme (C120 sg RNA)
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSADuet148

    Plasmid

    #121910

    Purpose

    Charge-introduced NpuDnaBΔ283ΔC39 intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    CI-NpuDnaBΔ290ΔC39 intein
    

    Tags

    His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 290-residue endonuclease domain, I53K…

    Promoter

    T7

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHBDuet259

    Plasmid

    #121911

    Purpose

    NpuDnaBΔ283ΔC39 intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    NpuDnaBΔ283ΔC39 intein
    

    Tags

    His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 283-residue endonuclease domain

    Promoter

    T7

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHBDuet116

    Plasmid

    #121915

    Purpose

    NpuDnaBΔ290ΔC39 intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    Oth-NpuDnaBΔ290ΔC39 intein
    

    Tags

    His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 290-residue endonuclease domain, I53K…

    Promoter

    T7

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6CUI (BRD2)

    Plasmid

    #119963

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    BRD2 (BRD2 Human)
    

    Tags

    GST tag followed by a long linker and TEV cleavag…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. 6DUB (LOC149281)

    Plasmid

    #119967

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    LOC149281 (METTL11B Human)
    

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 58-278

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pMLS415

    Plasmid

    #114272

    Purpose

    SapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

    Insert

    Cbr-Unc-119
    

    Use

    CRISPR and Cre/Lox

    Mutation

    *please see depositor comment below

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc-gamma1 (in pCAGGS)

    Plasmid

    #118039

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FOXN1 (FOXN1A-c017)

    Plasmid

    #110249

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FOXN1 (FOXN1 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LIMK2 (LIMK2A-c052)

    Plasmid

    #110264

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    LIMK2 (LIMK2 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJExpress414_GmPOPB-R663K

    Plasmid

    #92239

    Purpose

    Express R663K mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-R663K mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Arginine 663 to Lysine

    Promoter

    T7 promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2QO2 (EPHA3)

    Plasmid

    #101271

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EPHA3 (EPHA3 Human)
    

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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