We narrowed to 25,057 results for: Nes

1. pXEE401BG

   Plasmid

   #91717

   Purpose

   CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM441

   Plasmid

   #61271

   Purpose

   Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

   Plasmid

   #171796

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDM8 hCD81

   Plasmid

   #11588

   Depositor

   Shoshana Levy

   Article

   Oren et al Mol Cell Biol. 1990 Aug . 10(8):4007-15.

   Insert

   CD81 (CD81 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pH6HTC_RBFOX2-Halo

   Plasmid

   #175304

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   RBFOX2 (RBFOX2 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-scrCdh20.1 GFP

   Plasmid

   #209106

   Purpose

   GFP expressing scramble of shRNA targeting Cdh20

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCdh20.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJMP8930

   Plasmid

   #220905

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA.PKD.K/W

   Plasmid

   #10809

   Depositor

   Alex Toker

   Article

   Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

   Insert

   PKD (PRKD1 Human)
   

   Use

   Tags

   HA and Myc (inserted between amino acids 33&3…

   Expression

   Mammalian

   Mutation

   Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

   Promoter

   CMV

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5/FRT/TO HSPA6

    Plasmid

    #19459

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA6 (HSPA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-HsRRP12_K

    Plasmid

    #146766

    Purpose

    Mammalian Expression of HsRRP12

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsRRP12 (RRP12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

    Plasmid

    #213119

    Purpose

    Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Promoter

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMGS58

    Plasmid

    #135311

    Purpose

    A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    HygR-P2A-AID
    

    Use

    N terminal tagging plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ210

    Plasmid

    #109329

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLv-CMV-MyoD

    Plasmid

    #26808

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    MyoD (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJEP205-AAV-TRE3G-GFP-pA

    Plasmid

    #164789

    Purpose

    AAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQTEV-RPL9

    Plasmid

    #31334

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    ribosomal protein L9 (RPL9 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FBL6-FLAG3C

    Plasmid

    #19904

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    FBL6 (FBXL6 Human)
    

    Use

    Tags

    FLAG3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-mFzd10

    Plasmid

    #42262

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 10 (Fzd10 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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