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We narrowed to 8,085 results for: 104

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Showing: 1121 - 1140 of 8085 results

  1. pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54)

    Plasmid
    #242671
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + G1104K mutations in SpCas9(G1104K/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242671-plasmid-map-sequence-id-488983

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  15. pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

    Plasmid
    #160631
    Purpose
    Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.
    Depositor
    Diego Orzaez
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U626
    Available Since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160631-plasmid-map-sequence-id-321967

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  16. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  18. JPZF1 CMV-TO-SCN1A-377(G2E, A104K, S109I)-deImmLink-NZF

    Plasmid
    #236153
    Purpose
    Express mutated SCN1A-14 zinc finger array (G2E, A104K, S109I) attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    SCN1A-377-deImmLink-NZF
    Expression
    Mammalian
    Mutation
    G2E, A104K, S109I in SCN1A-377
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236153-plasmid-map-sequence-id-468410

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  20. pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)

    Plasmid
    #155134
    Purpose
    Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b
    Depositor
    Sinisa Urban
    Article
    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910
    Insert
    GlpG (glpG Escherichia coli)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …
    Promoter
    T7
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155134-plasmid-map-sequence-id-310516

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Showing: 1121 - 1140 of 8085 results