We narrowed to 13,540 results for: 109

1. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

   Plasmid

   #70195

   Purpose

   Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   tetR-deGFP fusion
   

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

   Plasmid

   #103869

   Purpose

   dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

   Plasmid

   #199362

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOstar-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIneo-V5-SBP-MBP-HsNot4_109-189-GSSG-275-575_AG

   Plasmid

   #148767

   Purpose

   Mammalian Expression of HsNot4_109-189-GSSG_275-575

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4_109-189-GSSG_275-575 (CNOT4 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W

   Plasmid

   #147887

   Purpose

   Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres

   Depositor

   Elisa Izaurralde

   Article

   Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

   Insert

   HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres (CNOT1 Human)
   

   Expression

   Mammalian

   Mutation

   1 silent mutation compared to the sequence of iso…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBS1109 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD]

   Plasmid

   #107841

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal domain in splicing reporter

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

   Plasmid

   #103871

   Purpose

   dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159673

   Purpose

   Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
   

   Tags

   T2A-GFP

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. JPZF1 CMV-TO-SCN1A-377(G2E, A104K, S109I)-deImmLink-NZF

   Plasmid

   #236153

   Purpose

   Express mutated SCN1A-14 zinc finger array (G2E, A104K, S109I) attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   SCN1A-377-deImmLink-NZF
   

   Expression

   Mammalian

   Mutation

   G2E, A104K, S109I in SCN1A-377

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)

    Plasmid

    #39303

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Insert

    EB1 (MAPRE1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q109A, W110A, K112A and K113A

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184740

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. JM109

    Bacterial Strain

    #49761

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    None

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-Ef1a-DIO-PPO-Venus (AAV9)

    Viral Prep

    #139505-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Promoter

    EF1a

    Tags

    Venus (Cre-dependent)

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-Ef1a-DIO-PPO-Venus (AAV8)

    Viral Prep

    #139505-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Promoter

    EF1a

    Tags

    Venus (Cre-dependent)

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CAG-DIO-PPO-Venus (AAV5)

    Viral Prep

    #139504-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-DIO-PPO-Venus (#139504). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-PPO-Venus plasmid DNA. CAG-driven, Cre-dependent expression of Venus-tagged parapinopsin. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Promoter

    CAG

    Tags

    Venus

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

17. pKSS

    Plasmid

    #39272

    Depositor

    Peter Kast

    Article

    Kast et al Gene. 1994 Jan 28;138(1-2):109-14.

    Type

    Empty backbone

    Use

    Positive-selection cloning vector

    Expression

    Bacterial

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Recall-miniCMV-sfGFP

    Plasmid

    #137995

    Purpose

    Backbone for generation of ClonMapper Recall Plasmid

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5XDRE-luciferase

    Plasmid

    #85623

    Purpose

    Luciferase reporter containing five tandem copies of the core Dpp response element

    Depositor

    Kenneth Irvine

    Article

    Oh et al Dev Cell. 2011 Jan 18;20(1):109-22. doi:

    Insert

    5x DRE
    

    Use

    Luciferase

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA.PKD

    Plasmid

    #10808

    Depositor

    Alex Toker

    Article

    Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

    Insert

    PKD (PRKD1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L520P

    Promoter

    CMV

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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