We narrowed to 11,794 results for: 11

1. pRL-TK 4x mut E

   Plasmid

   #11318

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   4 bulged binding sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Mutation E (see paper) on inner 2 binding sites.

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pRL-TK 4x mut F

   Plasmid

   #11319

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   4 bulged binding sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Mutation F (see paper) on inner 2 binding sites.

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pRL-TK 4x mut H

   Plasmid

   #11321

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   4 bulged binding sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Mutation H (see paper) on inner 2 binding sites.

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pRL-TK 4x mut GFP

   Plasmid

   #11323

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pRB327

   Plasmid

   #8668

   Depositor

   David Botstein

   Article

   Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

   Insert

   TUB1 (TUB1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 30, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pRB2065

   Plasmid

   #8669

   Depositor

   David Botstein

   Article

   Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

   Insert

   TUB1 upstream of LEU2 (TUB1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 30, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pRB2067

   Plasmid

   #8670

   Depositor

   David Botstein

   Article

   Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

   Insert

   TUB1 upstream of HIS3 (TUB1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 30, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pJBL2191

   Plasmid

   #71205

   Purpose

   pbuE STAR Antisense Expression Plasmid

   Depositor

   Julius Lucks

   Article

   Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

   Insert

   pbuE STAR Antisense
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJBL2184

   Plasmid

   #71209

   Purpose

   pbuE Sense Expression Plasmid

   Depositor

   Julius Lucks

   Article

   Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

   Insert

   pbuE Sense
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJBL1864

    Plasmid

    #71210

    Purpose

    Anti-anti Sense Expression Plasmid

    Depositor

    Julius Lucks

    Article

    Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

    Insert

    Anti-anti Sense
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMT Draper-GFP

    Plasmid

    #126653

    Purpose

    Inducible expression of Draper-GFP in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMT Crk-mCherry

    Plasmid

    #126659

    Purpose

    Inducible expression of Crk-mCherry in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Crk
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMT vCrk-GFP

    Plasmid

    #126661

    Purpose

    Inducible expression of vCrk truncation in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Crk
    

    Use

    Tags

    Expression

    Insect

    Mutation

    residues 1-182

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT Ptp69D-GFP

    Plasmid

    #126663

    Purpose

    Inducible expression of Ptp69D-GFP in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Ptp69D
    

    Use

    Tags

    Expression

    Insect

    Mutation

    A53S, K105N, and A98T- please see depositor comme…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT Shark-mCherry

    Plasmid

    #126668

    Purpose

    Inducible expression of Shark-mCherry in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Shark mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET His10-Draper INT

    Plasmid

    #126670

    Purpose

    Expression of His10-Draper intracellular domain for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper cytoplasmic domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid

    #67277

    Purpose

    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Inserts

    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    

    Use

    Tags

    mTQ2 blue fluorescent and red flourescent gene ka…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1.Gα11-LgB97

    Plasmid

    #134361

    Purpose

    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα11-LgB97 (GNA11 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-EGFP-C1-Hs4E-T_AF

    Plasmid

    #148687

    Purpose

    Mammalian Expression of Hs4E-T

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    Hs4E-T (EIF4ENIF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFPN1-N-gamma-tubulin (334-449)

    Plasmid

    #87859

    Purpose

    Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment from aa 334 to 449

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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