Addgene

We narrowed to 2,257 results for: Beta-s

Showing: 1121 - 1140 of 2257 results

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H513 px330.sgActin.UTR.2

Plasmid

#138176

Purpose

CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

Depositor

Wen Xue

Article

Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

Insert

sgActin (Actb Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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ADRB2-DuET

Plasmid

#213181

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADRB2 (ADRB2 Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGBT-R16

Plasmid

#31827

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

mini Tol2 ITR

Use

Cre/Lox; Gene trap

Tags

mRFP

Expression

Mutation

mRFP lacks start codon

Promoter

beta Actin

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B

Plasmid

#31439

Depositor

Gerhart Ryffel

Article

Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX p85b(NC)-SH2

Plasmid

#46468

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(NC) (PIK3R2 Bovine)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-717

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-V159N

Plasmid

#118383

Purpose

Gateway compatible donor vector with actin-V159N.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

V159N

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B_P328L329del

Plasmid

#30528

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

P328L329delCCTCT

Promoter

Available since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-G168D,Y169D

Plasmid

#118384

Purpose

Gateway compatible donor vector with actin-G168D,Y169D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

G168D,Y169D

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins

Plasmid

#31440

Depositor

Gerhart Ryffel

Article

Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

A263insGG

Promoter

Available since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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MZB053

Plasmid

#217835

Purpose

Bacterial expression of human AP3B1 (1-677) and AP3M1 AP-3 core hemicomplex

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Inserts

AP3B1 (AP3B1 Human)
AP3M1 (AP3M1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Removed residues 678-1094 from AP3B1

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP32

Plasmid

#196770

Purpose

Contains RBOHF promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

RESPIRATORY BURST OXIDASE HOMOLOG F (RBOH F Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2075

Plasmid

#158098

Purpose

High Copy number plasmid containing smCBA insert.

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

smCBA

Use

Tags

Expression

Mutation

Promoter

smCBA

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAPM-miR30-TMEM173-ts1

Plasmid

#174283

Purpose

TMEM173 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

TMEM173 shRNA (STING1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-TMEM173-ts2

Plasmid

#174284

Purpose

TMEM173 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

TMEM173 shRNA (STING1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-TMEM173-ts3

Plasmid

#174285

Purpose

TMEM173 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

TMEM173 shRNA (STING1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ADRB1-DuET

Plasmid

#213180

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADRB1 (ADRB1 Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS W391A pcDNA3

Plasmid

#206077

Purpose

Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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QuasAr-P2A-hKvb1

Plasmid

#154083

Purpose

Independent expression of QuasAr and human Kvbeta 1 in pFCK vector

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

QuasAr-P2A-human Kvb1 (KCNAB1 Synthetic, Human)

Use

Lentiviral

Tags

myc-Flag

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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gh186

Plasmid

#106847

Purpose

expression of gRNA targeting B3GNTL1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GNTL1 (B3GNTL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR63

Plasmid

#106029

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR63; Opto-GPR63; C7)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR63 (GPR63 Bovine, Human)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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