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Showing: 1121 - 1140 of 39071 results

  1. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Expression
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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  5. pCMV6-AN-HA_aa 1-542 POLK

    Plasmid
    #201585
    Purpose
    Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    Polymerase kappa (POLK Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Expresses amino acids 1-542 of DNA polymerase kap…
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201585-plasmid-map-sequence-id-395030

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  8. pTH862-CEN-slowmaxRLuc/minCFLuc

    Plasmid
    #115371
    Purpose
    Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase
    Depositor
    Tobias von der Haar
    Inserts
    Firefly Luciferase (codon minimised)
    Gcn4 5'-UTR + Renilla luciferase (codon optimised)
    Use
    Tags
    Expression
    Yeast
    Mutation
    All codons have been changed for the most favoura…
    Promoter
    ADH1 and TDH3
    Available since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    115371-plasmid-map-sequence-id-247306

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  10. MG1655-tMMR

    Bacterial Strain
    #62392
    Purpose
    E. coli MG1655 temperature-sensitive mismatch repair deficient strain, harboring mutS(A134V) mutL(G62S), and nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} for λ Red expression
    Depositor
    Csaba Pál
    Article
    Nyerges et al Nucleic Acids Res. 2014 Apr;42(8):e62. d
    Bacterial Resistance
    Tetracycline
    Species
    Escherichia coli
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62858-plasmid-map-sequence-id-243538

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  17. pJRM919_WT aa 1-419 His POLI

    Plasmid
    #201445
    Purpose
    Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    DNA polymerase iota (POLI Human)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Expresses amino acids 1-449 of DNA polymerase iot…
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201445-plasmid-map-sequence-id-395334

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Showing: 1121 - 1140 of 39071 results