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Plasmid#80391Purposeexpression copGFP and Puromycine, with an S/MAR element for episomal maintainceDepositorInsertcopGFP-T2A-Puromycine
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Neo
Plasmid#48336PurposeTrasgenically expresses firefly luciferase in T. cruzi with neomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceJuly 20, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
psiCHECK2-2A-3xFLAG-XBP1u_pause-2A
Plasmid#159583PurposeLuciferase reporter for ribosome pausingDepositorInsertsXBP1u pause site
EIF2S3 5UTR
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-2A-3xFLAG-SBP-2A
Plasmid#159584PurposeLuciferase reporter for ribosome pausing controlDepositorInserts2A-3xFLAG-SBP-2A
EIF2S3 5UTR
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBB HA cIAP1
Plasmid#38232DepositorInsertcIAP1 (BIRC2 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDSW1728
Plasmid#120812PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)DepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
TPC2-G-GECO1.2
Plasmid#207141PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mCherry
Plasmid#169411PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherryDepositorInsertspLeft
mCherry
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc double blue
Plasmid#170050PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blueDepositorInsertsmTagBFP2
CO BFP
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pTcpP-mCherry
Plasmid#192861PurposepMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917DepositorInsertTcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc double green
Plasmid#169412PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double greenDepositorInsertsmWasabi
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc triple red
Plasmid#169413PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple redDepositorInsertsmScarlet proper
COmScarlet
mRuby3
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-EYFP-Kv
Plasmid#89256PurposeChannelrhodopsin localized to neuronal soma via a Kv2.1 targeting motifDepositorInsertChannelrhodopsin
UseAAVTagsEYFPExpressionMammalianMutationH134RPromotersynapsinAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorTagsExpressionBacterial and MammalianMutationPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrex-n-eGFP
Plasmid#62544PurposeExpression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genomeDepositorInserteGFP
Use(1)trypanosoma cruzi expression of egfp (2) genom…TagsNoneExpressionMutationNonePromoterRibo-HX1Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrex-b-NLS-hSpCas9
Plasmid#62543PurposeExpression of Cas9 in T. cruziDepositorInsertNLS-hSpCas9
UseCRISPR; Trypanosoma cruzi expressionTags3xFLAG and NLSExpressionMutationPromoterRibo-HX1Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Px
Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
UseTags10 aa peroxisome targeting signal 1 (IHHPRELSRL) …ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only