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M_NLS 67kDa

Plasmid

#201351

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-6PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Arp2_pLib

Plasmid

#173676

Purpose

Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp2 (ACTR2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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HNRNPCL1_RRM_pGEX

Plasmid

#135116

Purpose

Recombinant expression of HNRNPCL1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

HNRNPCL1 (HNRNPCL1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1 K137R

Plasmid

#85694

Purpose

Mammalian expression of mouse GATA1 with K137R mutation

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Mutation

K137R (SUMO-1 attachment site)

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
Arp3_pLib

Plasmid

#173677

Purpose

Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp3 (ACTR3 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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RBMS3_2xRRM_pGEX

Plasmid

#135142

Purpose

Recombinant expression of RBMS3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS3 (RBMS3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 10, 2021

Availability

Academic Institutions and Nonprofits only

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RBM24_RRM_pGEX

Plasmid

#135148

Purpose

Recombinant expression of RBM24 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM24 (RBM24 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19-NLS-4

Plasmid

#54222

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 NLS (PCNA Human)

Tags

SV40NLS and mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS

Plasmid

#43969

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Gal4/DBD

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19-SV40NLS-4

Plasmid

#54223

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

3xSV40NLS and mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

Plasmid

#200877

Purpose

ZooTsy_TnsF expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_TnsF

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT315: pHelper(ZooTsy)_TnsF-Y584F

Plasmid

#200872

Purpose

Inducible expression of ZooTsy proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT155: pHelper(ZooTsy)_ΔHTH

Plasmid

#200868

Purpose

Inducible expression of ZooTsy (except HTH) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT156: pHelper(ZooTsy)_ΔTnsF

Plasmid

#200869

Purpose

Inducible expression of ZooTsy (except TnsF) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT154: pHelper(ZooTsy)_ΔYRec

Plasmid

#200867

Purpose

Inducible expression of ZooTsy (except YRec) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF

Plasmid

#200865

Purpose

AjTn6022_TniQ expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022_TnsF

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT401: pHelper(AjTn6022)_QF-separated_TniQ-K361D

Plasmid

#200861

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Mutation

TniQ-K361D

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT400: pHelper(AjTn6022)_QF-separated_TniQ-K348D

Plasmid

#200860

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Mutation

TniQ-K348D

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT151: pHelper(AjTn6022)_ΔTnsC

Plasmid

#200854

Purpose

Inducible expression of AjTn6022 proteins (except TnsC).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT152: pHelper(AjTn6022)_ΔTniQ

Plasmid

#200855

Purpose

Inducible expression of AjTn6022 proteins (except TniQ).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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