We narrowed to 28,721 results for: sta

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pRK5-Ufm1 ∆C3

Plasmid

#133866

Purpose

Expression of human UFM1 mutant lacking the last three C-terminal residues

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Plasmid

#158967

Purpose

Ubiquitous expression of mtON in C. elegans

Depositor

Andrew Wojtovich

Article

Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

Insert

eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Tags

GFP

Expression

Worm

Promoter

eft-3

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Plasmid

#219782

Purpose

CaST-IRES for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD/His-B-iRFP

Plasmid

#31855

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Expression

Bacterial

Mutation

S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

Promoter

araBAD

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-hfYFP

Plasmid

#191201

Purpose

Constitutive CAG-driven expression of hfYFP in animals using AAV

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

AAV

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CAG

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAB076i3

Plasmid

#79198

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pSum-LacZ-gfp

Plasmid

#109285

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

LacZalpha

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB180

Plasmid

#80677

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Type

Empty backbone

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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AA177

Plasmid

#215983

Purpose

Fragmid fragment: (N' terminus) Start codon only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1DA

Plasmid

#35028

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DA (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

D181A--NUCLEASE DEAD POINT MUTANT

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pCAG_mRuby2_smFP OLLAS

Plasmid

#59761

Purpose

encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_OLLAS

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3-del5

Plasmid

#44732

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

Maternally Expressed Gene 3 (MEG3 Human)

Expression

Mammalian

Mutation

The first 450 nt were deleted from the MEG3 cDNA.

Promoter

hCMV

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pSMART-sgRNA (Sp)

Plasmid

#80427

Purpose

U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJH3830

Plasmid

#200038

Purpose

Prig-3 zif-1 SL2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of zif RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ZIF (zif-1 Nematode)

Tags

mCherry

Expression

Worm

Promoter

Prig-3

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-TFR-20

Plasmid

#55327

Purpose

Localization: Transferin Receptor, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

TFR (TFRC Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pXPR_003 sgNFkB2 guide 1

Plasmid

#193593

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 1 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1deltaP

Plasmid

#35030

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DELTAP (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

8 AMINO ACID DELETION OF P BOX (amino acids 337–3…

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
mCherry-CyclinF

Plasmid

#32975

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

Flag and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only