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LDB-14-p65

Plasmid

#158985

Purpose

Expresses LDB-14 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-14-p65

Tags

V5 tag , 6xHis tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL1-FLAG

Plasmid

#15818

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin1 (CUL1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N452

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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p201H Cas9

Plasmid

#59176

Purpose

Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
hph

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET-His-SCov2-nsp8

Plasmid

#154758

Purpose

Expression of SARS-CoV-2 nsp8

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)

Plasmid

#104119

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

CheRiff-CFP

Use

Adenoviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-Rac1

Plasmid

#12200

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

Starting methionine (M1) removed; F78S compared t…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB4

Plasmid

#187768

Purpose

MAC-tagged gene expression of human EPHB4

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB4 (EPHB4 Human)

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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KillerOrange /pQE-30

Plasmid

#74748

Purpose

KillerOrange gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

Insert

KillerOrange

Tags

HisTag

Expression

Bacterial

Mutation

KillerRed with substitutions G3C Y66W D113S N145S…

Promoter

T5

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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SHC003BSD

Plasmid

#133300

Purpose

The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

Depositor

Jingshi Shen

Article

Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV

Plasmid

#235547

Purpose

To express GFP

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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p2H12Matry

Plasmid

#221143

Purpose

cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen-Matry

Expression

Bacterial

Promoter

Ptet

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pFLAG-4EHP

Plasmid

#17342

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
EGFP-mCherry-Ki67

Plasmid

#183750

Purpose

Expresses EGFP-mCherry-Ki67

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA14 (MSW#503)

Plasmid

#208069

Purpose

Expression of GFP-tagged hsRPA3 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA3 (RPA3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/cmycDrosha

Plasmid

#10828

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

Drosha (DROSHA Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)

Plasmid

#191451

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

N-WASP

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTK82_GFP-4.1G

Plasmid

#46353

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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MTK0_002

Plasmid

#123960

Purpose

Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - 2xHS4 - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL2-FLAG

Plasmid

#15819

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin2 (CUL2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N427

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pEN396-pCAGGS-TIR1-V5-2A-HygroR

Plasmid

#195102

Purpose

Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

TIR1

Tags

V5

Expression

Mammalian

Promoter

pCAGGS

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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