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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,573 results for: his

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Showing: 11481 - 11500 of 23573 results

  10. TEVEp_pRK793

    Plasmid
    #247672
    Purpose
    pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVEp insert based on Yi et al., 2013.
    Depositor
    Carl Denard
    Article
    Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2
    Insert
    MBP-ENLYFES-7xHIS-TEVEp
    Tags
    His and MBP
    Expression
    Bacterial
    Mutation
    S120R, D148R, T173A, N177K, M218I, S219P
    Available Since
    Feb. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    247672-plasmid-map-sequence-id-491563

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  12. pTR01F/hFGFR3c-N540K-V5

    Plasmid
    #246390
    Purpose
    for PiggyBac mediated integration and stable expression of hFGFR3c-N540K protein, mutant variant causes Hypochondroplasia and Thanatophoric dysplasia
    Depositor
    Pavel Krejčí
    Article
    Ursachi et al J Bone Miner Res. 2026 Jan 26:zjag017. d
    Insert
    human FGFR3c-N540K (FGFR3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    N540K substitution
    Promoter
    truncated CMV promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246390-plasmid-map-sequence-id-491100

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  16. MZB131

    Plasmid
    #217839
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    AP3D1 (AP3D1 Human)
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Deleted residues 1-919
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217839-plasmid-map-sequence-id-432995

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  17. MZB150

    Plasmid
    #217840
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    ARNO (CYTH2 Human)
    Tags
    10x His and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Deleted residues 1-51 and 253-400
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217840-plasmid-map-sequence-id-432997

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  18. FLuc-C-HiBiT

    Plasmid
    #207118
    Purpose
    Bacterially expressed FLuc-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    luciferin 4-monooxygenase
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207118-plasmid-map-sequence-id-417161

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Showing: 11481 - 11500 of 23573 results