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pJ211_rha_WT_low

Plasmid

#128851

Purpose

Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pD441_WT_hi

Plasmid

#128857

Purpose

High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMiniT:GFP-Gephyrin TKIT donor

Plasmid

#169433

Purpose

Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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EC70208

Plasmid

#209469

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70198

Plasmid

#209459

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70200

Plasmid

#209461

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.23-MYC-e5

Plasmid

#120343

Purpose

pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

chr8:130594100-130596043 (hg19)

Use

Luciferase

Expression

Mammalian

Promoter

MYC

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23-MYC-e4

Plasmid

#120342

Purpose

pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

chr8:129060477-129062593 (hg19)

Use

Luciferase

Expression

Mammalian

Promoter

MYC

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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EC70207

Plasmid

#209468

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1238 PINK1-mCherry-FIREtag

Plasmid

#205174

Purpose

Expresses PINK1-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

PINK1-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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zbFGF

Plasmid

#12309

Depositor

James Thomson

Article

Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

Insert

Fgf2 (fgf2 Zebrafish)

Expression

Bacterial

Mutation

Ala 11 mutated to Pro, Ser 55 mutated to Asn

Available Since

Sept. 25, 2006

Availability

Academic Institutions and Nonprofits only

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mSA-phi29

Plasmid

#210815

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

mSA-Phi29

Expression

Bacterial

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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mEmerald-ERBB2-N-18

Plasmid

#62755

Purpose

Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509

Depositor

Michael Davidson

Insert

ERBB2 (ERBB2 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMU-TGN41r

Plasmid

#61176

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T1 (+minintr)

Plasmid

#119204

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 1 mini-gene (NF1 Human)

Use

Transfer vector

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Expression

Plant

Mutation

D156R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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EC70195

Plasmid

#209456

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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