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PAC_E83_LOV_eGFP

Plasmid

#231574

Purpose

This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Insert

PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBsVchCAST-pyrE

Plasmid

#203813

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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peGFP C1-SPG20 SC domain

Plasmid

#218580

Purpose

Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Mutation

deleted amino acids 1-417

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-LIN-41

Plasmid

#52716

Purpose

retroviral expression of human LIN-41 / TRIM71

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV3FLAG_hGTPBP4

Plasmid

#53974

Purpose

Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

GTPBP4 (GTPBP4 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pRR-ERbeta-5Z

Plasmid

#23062

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERbeta (ESR2 Human)

Expression

Yeast

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
pGEM-T_mouseH3.1-HaloTag

Plasmid

#244242

Purpose

CRISPR/Cas9 donor plasmid to tag mouse histone H3.1 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

H3.1 (H3c1 Mouse)

Use

CRISPR

Tags

HaloTag

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-Calnexin-N-14

Plasmid

#158004

Purpose

Visualization of ER in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Calnexin (CANX Human)

Tags

mGold

Expression

Mammalian

Mutation

I474N

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pdCASclos

Plasmid

#73640

Purpose

Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

dCas9
sgRNA to spo0A

Use

E.coli-clostridium shuttle vector

Mutation

D10A, H840A

Promoter

Pj23119 and ptb

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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bArr2-V5

Plasmid

#201494

Purpose

Expression of the beta-arrestin2 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr2-V5 (ARRB2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-N90-beta-catenin

Plasmid

#31787

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Use

Gateway Cloning

Tags

Myc

Mutation

Deletion of aa 1-90- Constitutively Active

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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cjBlue chromoprotein

Plasmid

#117844

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, cjBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Tagger

Plasmid

#129396

Purpose

Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

Insert

rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2

Tags

FLAG, HA, and V5

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

Plasmid

#163025

Purpose

Expression of CRISPRi with gRNA against mouse Pcsk9

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Promoter

Alb-AAT

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMy-CTCF-GFP

Plasmid

#50574

Purpose

Retroviral vector endoding for mouse CTCF fused to GFP

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pyc111f

Plasmid

#158780

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6f-RPL10a

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-CRY2-VHH(GFP)

Plasmid

#58370

Purpose

For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

SNAP and vhhGFP4

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(A)

Plasmid

#87913

Purpose

Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499A

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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