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LCV2_PSMB2_sgRNA_1

Plasmid

#155092

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene)

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMB2_sgRNA_1 (PSMB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_p.D162E

Plasmid

#81732

Purpose

Gateway Donor vector containing , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

D162E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_p.R535Q

Plasmid

#81713

Purpose

Gateway Donor vector containing CTNNB1,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

R535Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_p.H36Y

Plasmid

#81690

Purpose

Gateway Donor vector containing CTNNB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

H36Y

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_p.I35S

Plasmid

#81677

Purpose

Gateway Donor vector containing CTNNB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

I35S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MDM2_p.E421Q

Plasmid

#82876

Purpose

Gateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MDM2 (MDM2 Human)

Use

Gateway entry vector

Mutation

E421Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TRCN0000297275

Plasmid

#78152

Purpose

shCDK4 targeting 3UTR

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223_FBXO15_WT

Plasmid

#81839

Purpose

Gateway Donor vector containing FBXO15 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXO15 (FBXO15 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

Plasmid

#156405

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_ISX_WT

Plasmid

#81849

Purpose

Gateway Donor vector containing ISX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ISX (ISX Human)

Use

Gateway entry vector

Mutation

S28G; P57S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_B2M_p.A6V

Plasmid

#81263

Purpose

Gateway Donor vector containing B2M , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

B2M (B2M Human)

Use

Gateway entry vector

Mutation

A6V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.T142M

Plasmid

#81525

Purpose

Gateway Donor vector containing KEAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

T142M; C241F; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.R258H

Plasmid

#81581

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway entry vector

Mutation

R258H; p.588_589delLA-insRL

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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px330-Apob-2

Plasmid

#162549

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-1

Plasmid

#162548

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-3

Plasmid

#162550

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-4

Plasmid

#162551

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_COPS2_p.A317D

Plasmid

#81434

Purpose

Gateway Donor vector containing COPS2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

COPS2 (COPS2 Human)

Use

Gateway entry vector

Mutation

A317D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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BAP1-C91A

Plasmid

#108440

Purpose

Stable expression of BAP1-C91A in mammalian cells.

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

Insert

BAP1 (BAP1 Human)

Expression

Mammalian

Mutation

C91A: c.271T>G, c.272G>C

Promoter

CMV

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_p.G31V

Plasmid

#81518

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway entry vector

Mutation

G31V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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