168,321 results

1. pYPQAT57

   Plasmid

   #223429

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdTomato-anti-AlfaTag nanobody

   Plasmid

   #171819

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   tdTomato-anti-AlfaTag nanobody
   

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-ER-mRFP

   Plasmid

   #231180

   Purpose

   Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vector

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   ER-mRFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-puro Ras V12

   Plasmid

   #1768

   Depositor

   Bob Weinberg

   Insert

   ras V12 (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G12V, constitutively active

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRVL-2

   Plasmid

   #104580

   Purpose

   Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Mouse IgG2c CH1/CH2/CH3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rogi1-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179864

   Purpose

   Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV SYN flex PSAM4 GlyR IRES EGFP (AAV9)

   Viral Prep

   #119741-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV SYN flex PSAM4 GlyR IRES EGFP (#119741). In addition to the viral particles, you will also receive purified AAV SYN flex PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven, Cre-dependent expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Promoter

   Syn

   Tags

   EGFP (Cre-dependent)

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-SNAP29

   Plasmid

   #45915

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   synaptosomal-associated protein, 29kDa (SNAP29 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-Cas9-Act3.0

   Plasmid

   #178954

   Purpose

   It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_EF1a_Flag_EGFP

    Plasmid

    #183117

    Purpose

    Expresses flag-tagged EGFP in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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