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pDB2-hCRY2-Luciferase-CD4-bla

Plasmid

#189985

Purpose

Donor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Luciferase

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(HA)

Plasmid

#195023

Purpose

HA-tagged Creb5 in pInducer20 lentivirus destination vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 128)

Plasmid

#195031

Purpose

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G137I)

Plasmid

#133272

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Use

Oocyte expression

Mutation

G137I

Promoter

T7

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W

Plasmid

#208552

Purpose

Lentiviral vector expressing gRNA targeting human RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX3(13) (RUNX3 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-W

Plasmid

#208551

Purpose

Lentiviral vector expressing gRNA targeting human RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX3(12) (RUNX3 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(AAVS1)-PGKpuro2ABFP-W

Plasmid

#200459

Purpose

Lentiviral vector expressing gRNA targeting human AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W

Plasmid

#210612

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(1)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W

Plasmid

#200491

Purpose

Lentiviral vector expressing gRNA targeting human RIC8A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RIC8A(16) (RIC8A Human)

Use

Lentiviral

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W

Plasmid

#200490

Purpose

Lentiviral vector expressing gRNA targeting human RIC8A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RIC8A(15) (RIC8A Human)

Use

Lentiviral

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

Plasmid

#210613

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(2)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-Δ220-234-Flag

Plasmid

#210341

Purpose

Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Δ220-234

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-H120N/F121L-Flag

Plasmid

#210340

Purpose

Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

H120N/F121L

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-FR-2Strep

Plasmid

#210316

Purpose

Expresses SARS-CoV-2 NSP4 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-mCherry

Plasmid

#210319

Purpose

Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP10-2Strep

Plasmid

#210357

Purpose

Expresses FKBP10 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

FKBP10 (FKBP10 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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DNAJC11-2Strep

Plasmid

#210354

Purpose

Expresses DNAJC11 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

DNAJC11 (DNAJC11 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro

Plasmid

#210856

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

L324D/R543D/R548D

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (363-450)

Plasmid

#210849

Purpose

Mammalian expression of ATG13 (363-450) mutant

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13 (ATG13 Human)

Expression

Mammalian

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746-C571A

Plasmid

#207821

Purpose

N-terminal tagged ZNF746-C571A for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746-C571A (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Mutation

C571A

Promoter

UBC

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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