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pET28a-SUMO-SpyTag003

Plasmid

#216282

Purpose

Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SUMO-SpyTag003

Tags

His6 and SpyTag003

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHygO2

Plasmid

#200077

Purpose

hygR selection unmarked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA1

Plasmid

#199727

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA1 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA2

Plasmid

#199728

Purpose

Golden Gate entry vector; 2nd gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA2 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBL101gg

Plasmid

#199735

Purpose

Golden Gate recipient vector for transgene overexpression

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

sacB

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsLSm1_J

Plasmid

#146658

Purpose

Mammalian Expression of HsLSm1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsLSm1 (LSM1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_C_ROR2

Plasmid

#187759

Purpose

MAC-tagged gene expression of human ROR2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ROR2 (ROR2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-8e-E1347A

Plasmid

#187163

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-8e-E1347A

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJH625_pJH_LldR_PlldR-sfGFP.0TAG

Plasmid

#163724

Purpose

Lactate-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH674_pTech_PlldR-HHRz-supP

Plasmid

#163728

Purpose

Lactate-inducible expression of supP, preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 G10-90p

Plasmid

#153370

Purpose

Synthetic promoter for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

G10-90 promoter

Expression

Plant

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-Rad18UBD

Plasmid

#119010

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 ACT7p

Plasmid

#153371

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

ACT7 promoter

Expression

Plant

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 eCFP CDS

Plasmid

#153397

Purpose

Cyan fluorescent protein coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

eCFP CDS

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pME-QUAS5x:GFPNLS-SV40pA

Plasmid

#155115

Purpose

Middle entry vector containing QUAS5x element upstream of GFP-NLS.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x:GFPNLS

Use

Gateway middle entry vector

Promoter

QUAS5x-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-1.03kb-EGFP

Plasmid

#153164

Purpose

Truncated mouse gamma-synuclein (mSncg-1.03kb) promoter-mediates gene expression in mammalian retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-0.66kb-EGFP

Plasmid

#153165

Purpose

Truncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0663

Plasmid

#133478

Purpose

Expression in fission yeast from promoter pact1, integrated at his5 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-Dr-TrkA

Plasmid

#127963

Purpose

Myr mCherryDrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-mCherry-3xSAG-Dr-hel-4- TrkA

Expression

Mammalian

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-MaSgRNA_PuroR

Plasmid

#84780

Purpose

Expresses major satellite-specific sgRNA.

Depositor

Heinrich Leonhardt

Article

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Insert

major satellite-specific sgRNA

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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