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pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

Plasmid

#223141

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

Plasmid

#106466

Purpose

Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(S219A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Serine 219 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase delta 1278-1291

Plasmid

#59830

Purpose

Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 1278-1291

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

Plasmid

#63565

Purpose

Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
AiP12982 - pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2982)

Plasmid

#191709

Purpose

AI plasmid ALIAS: AiP12982. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12927 - pAAV-AiE0078m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2927)

Plasmid

#230365

Purpose

AiE0078m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12918 - pAAV-AiE0459m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2918)

Plasmid

#224016

Purpose

AiE0459m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12965 - pAAV-AiE0444h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2965)

Plasmid

#191729

Purpose

AI plasmid ALIAS: AiP12965. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatum

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mTFP1

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12944 - pAAV-AiE0667m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2944)

Plasmid

#224017

Purpose

AiE0667m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET_Chrna6 neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

Plasmid

#101211

Purpose

Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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AiP12924 - pAAV-AiE0078h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2924)

Plasmid

#229907

Purpose

AiE0078h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12946 - pAAV-AiE0697m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2946)

Plasmid

#230268

Purpose

AiE0697m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12923 - pAAV-AiE0078h_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2923)

Plasmid

#230441

Purpose

AiE0078h_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12926 - pAAV-AiE0078m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2926)

Plasmid

#230442

Purpose

AiE0078m_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12945 - pAAV-AiE0679m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2945)

Plasmid

#230267

Purpose

AiE0679m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12980 - pAAV-AiE0364h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2980)

Plasmid

#230272

Purpose

AiE0364h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12939 - pAAV-AiE0496m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2939)

Plasmid

#230263

Purpose

AiE0496m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12940 - pAAV-AiE0591m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2940)

Plasmid

#230264

Purpose

AiE0591m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12942 - pAAV-AiE0597m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2942)

Plasmid

#230265

Purpose

AiE0597m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12943 - pAAV-AiE0662m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2943)

Plasmid

#230266

Purpose

AiE0662m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge