Addgene

We narrowed to 1,214 results for: 5-Oct

Showing: 1141 - 1160 of 1214 results

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C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

Plasmid

#162958

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E44: attB5r-SNAP-nostop-attB1r

Plasmid

#162962

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E11: attB5r-RU5u-His6-nostop-attB1r

Plasmid

#162953

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

Plasmid

#162956

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E09: attB5r-RU5u-FLAG-nostop-attB1r

Plasmid

#162951

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

FLAG epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E59: attB5r-RU5u-2xHA-nostop-attB1r

Plasmid

#162966

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual HA epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E42: attB5r-RU5u-Strep2-nostop-attB1r

Plasmid

#162961

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E26: attB5r-RU5u-myc-nostop-attB1r

Plasmid

#162960

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Myc epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E10: attB5r-RU5u-HA-nostop-attB1r

Plasmid

#162952

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

HA epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E24: attB5r-RU5u-V5-nostop-attB1r

Plasmid

#162959

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

V5 epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-hAXL

Plasmid

#124797

Purpose

Tet-inducible AXL expression in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA_MET_D14_3Flag

Plasmid

#182495

Purpose

Contains cDNA of the MET gene without the exon 14

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

MET (MET Human)

Use

Tags

C-terminal 3xFlag tag

Expression

Mammalian

Mutation

Exon 14 deleted

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-c/o NL4-3 Env delta CT

Plasmid

#139285

Purpose

HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

codon optimized HIV-1 Env delta cytoplasmic tail

Use

Tags

Expression

Mammalian

Mutation

deletion of cytoplasmic tail after residue 710

Promoter

CMV IE

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pKIF1C-GFP

Plasmid

#130977

Purpose

Expression of KIF1C-GFP in mammalian cells.

Depositor

Anne Straube

Article

Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-

Insert

KIF1C-GFP (KIF1C Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

5 silent mutations that make this construct RNAi …

Promoter

CMV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-Brn4

Plasmid

#47028

Purpose

Expresses mouse Brn4

Depositor

Hans Schöler

Article

Han et al Cell Stem Cell. 2012 Apr 6;10(4):465-72.

Insert

Brn4 (Pou3f4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

The insert includes 40 bp of the TOPO vector in t…

Promoter

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb1

Plasmid

#162793

Purpose

Ccnb1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb1 shRNA (Ccnb1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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