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pME tdTomato-CAAX

Plasmid

#135204

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

tdTomato

Use

Synthetic Biology

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-HH1713

Plasmid

#124994

Purpose

E. coli expression of recombinant H. hepaticus antigen HH_1713

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH_1713

Expression

Bacterial

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKM492-Myc-His

Plasmid

#109283

Purpose

ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Myc-His

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX Tight Puro Snail

Plasmid

#101117

Purpose

Tet-On Snail

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

Snail cDNA

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pME turquoise2

Plasmid

#135207

Purpose

Cyan fluorescent tag

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mTurquoise2

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMyCA-mCherry

Plasmid

#84272

Purpose

mCherry fluorescence protein expression using M. smegmatis

Depositor

Young-Hwa Song

Article

Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

Insert

mCherry

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pYC1876

Plasmid

#158722

Purpose

Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

ssrA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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DDC_M_Tub_KDEL

Plasmid

#51529

Purpose

Ectopic expression of DDC with KDEL tag for CTAP

Depositor

Claus Jorgensen

Article

Tape et al Mol Cell Proteomics. 2014 May 12.

Insert

DDC

Tags

HA and KDEL

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pKM490-Clip

Plasmid

#109281

Purpose

ORBIT integrating plasmid for C-terminal tagging with CLIP tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-CLIP tag

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBEL2108

Plasmid

#201969

Purpose

ORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.

Depositor

Gira Bhabha, Damian Ekiert

Article

Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

Insert

attB-3C-eGFP-4xGly-TEV-Flag-6xHis

Use

Orbit integrating plasmid

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGMCKq1-10M1-sspBmyc

Plasmid

#27069

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

tetR-PmyctetO-sspB

Expression

Bacterial

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Kan)

Plasmid

#110089

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pME VIVIT NS

Plasmid

#188699

Purpose

Gateway middle element encoding the VIVIT peptide (no stop codon)

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VIVIT

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCOND (Apr)

Plasmid

#110094

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-mRFP

Plasmid

#27058

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-mcherry rfp

Expression

Bacterial

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Hyg)

Plasmid

#110090

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_CMVtrunc_877

Plasmid

#105828

Purpose

A modified pcDNA3.1- vector with reduced expression activity of a truncated CMV promoter

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Type

Empty backbone

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCOND (Hyg)

Plasmid

#110093

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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