We narrowed to 21,990 results for: ESC

1. pCapVQ329R

   Plasmid

   #183029

   Purpose

   the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28

   Depositor

   Ute Romling

   Article

   Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

   Insert

   patatin-like phospholipase variant CapVQ329R
   

   Use

   Tags

   No

   Expression

   Bacterial

   Mutation

   glutamine 329 changed to arginine

   Promoter

   pBAD promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB120

   Plasmid

   #167150

   Purpose

   MoClo-compatible Level 1 (position 1) vector encoding Kanamycin resistance cassette, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   pNOS-nptII-ocsT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pNOS

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBB0405-TEV-His12

   Plasmid

   #137040

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRevA-TEV-His12

   Plasmid

   #137034

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAF07416.1 (revA Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pP13-TEV-His12

   Plasmid

   #137035

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. eMscL G22S

   Plasmid

   #107455

   Purpose

   eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

   Depositor

   Francesco Difato

   Article

   Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

   Insert

   bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
   

   Use

   Adeno-associated viral

   Tags

   Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

   Expression

   Mammalian

   Mutation

   Bearing a glycine to serine substitution at posit…

   Promoter

   human synapsin 1

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG106 Lyn11-FAST

   Plasmid

   #130721

   Purpose

   Expresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   lyn11-FAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCA.66/2272

   Plasmid

   #22733

   Depositor

   Mark Magnuson

   Article

   Chen et al Dis Model Mech. 2011 Feb 14. ():.

   Insert

   Lox66-pU(delta)TK-EM7Neo-Lox2272
   

   Use

   Cre/Lox; Bac recombineering

   Tags

   Expression

   Mutation

   This vector was designed for assembling gene targ…

   Promoter

   Available since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG156 Mito-FAST

   Plasmid

   #130723

   Purpose

   Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   Mito-FAST
   

   Use

   Tags

   Myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG109 H2B-FAST

    Plasmid

    #130722

    Purpose

    Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    H2B-FAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pelB-MBP-BB0323-ss

    Plasmid

    #137067

    Purpose

    E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66700.1
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature BB0323: lacks the BB0323 signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTV001

    Plasmid

    #130265

    Purpose

    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

    Insert

    eatA::His8
    

    Use

    Tags

    polyhistidine (internal)

    Expression

    Bacterial

    Mutation

    8His polyhistidine encoding region and XhoI site …

    Promoter

    araBAD

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CHMP4C 156-233

    Plasmid

    #108257

    Purpose

    Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4C residues 156-233 (CHMP4C Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528 CHMP1A 140-196

    Plasmid

    #104593

    Purpose

    Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1A residues 140-196 (CHMP1A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCA528 CHMP2A 152-222

    Plasmid

    #104594

    Purpose

    Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A residues 152-222 (CHMP2A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 CHMP2B 141-213

    Plasmid

    #104595

    Purpose

    Expresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2B residues 141-213 (CHMP2B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA528 CHMP3 159-222

    Plasmid

    #104599

    Purpose

    Expresses C-terminal 159-222 residues of CHMP3 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-5

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP3 residues 159-222 (CHMP3 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pelB-MBP-BB0238-ss

    Plasmid

    #137046

    Purpose

    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature BB0238: lacks the BB0238 signal peptide

    Promoter

    T7lac

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pelB-MBP-P66-ss

    Plasmid

    #137045

    Purpose

    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66949.1 (p66 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature P66: lacks the P66 signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-3xFLAG-RSL1D1(WT)

    Plasmid

    #122301

    Purpose

    expresses RSL1D1 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RSL1D1 (RSL1D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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