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MsCLYBL_sgRNA1

Plasmid

#111294

Purpose

CRISPR KO murine CLYBL

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine CLYBL

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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XW99

Plasmid

#65842

Purpose

MosSCI-Pmig-13::QS::SL2::mCherry for ttTi4348 on Chrom I.

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Tags

mCherry

Expression

Worm

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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MsCLYBL_sgRNA6

Plasmid

#111295

Purpose

CRISPR KO murine CLYBL

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA6 against murine CLYBL

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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phpBCL2-FL(pA)

Plasmid

#42593

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pHelper_O

Plasmid

#173783

Purpose

The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

186 Integrase

Use

Synthetic Biology

Expression

Bacterial

Mutation

E59G - please see depositor comments

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_W

Plasmid

#173784

Purpose

The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Wphi Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_D

Plasmid

#173781

Purpose

The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

D145 Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLNCX2-lifeact-mAPPLE

Plasmid

#89820

Purpose

mAPPLE labeled fluorescent actin

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

lifeact-mAPPLE

Use

Retroviral

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEBG-GST-TRAF2

Plasmid

#44101

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Promoter

-

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pTORKm43GW

Plasmid

#112897

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418).

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128V

Plasmid

#61733

Purpose

Expresses human Y128V NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Mutation

Y128V

Promoter

Ef1a

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-mCherry-6H

Plasmid

#187104

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

mCherry

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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phpRL-BCL2-FL-pA

Plasmid

#42595

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1

Plasmid

#113943

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-CAR-GECO1

Plasmid

#113939

Purpose

Bacterial expression of red fluorescent Ca2+ sensor CAR-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

CAR-GECO1

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV myc DAF-12

Plasmid

#32566

Depositor

Al Fisher

Article

Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

Insert

DAF-12 (daf-12 Nematode)

Tags

myc epitope

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_sgRNA

Plasmid

#68422

Purpose

Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Gluc sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Ptrc::RpaA D53A

Plasmid

#105229

Purpose

inducible expression of unphosphorylatable variant of RpaA (D53A)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

rpaA driven by Ptrc promoter

Use

Cyanobacteria cloning vector

Mutation

D53 mutated to A

Promoter

ptrc

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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XW21

Plasmid

#65837

Purpose

XW21 Punc-4c-QS-3'UTR-unc-54 (no SL2-mcherry)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Expression

Worm

Promoter

Punc-4c

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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