We narrowed to 22,251 results for: mpl

1. pCAG-mDrc3-PA

   Plasmid

   #176467

   Purpose

   Expression vector of mouse dynein regulatory complex subunit 3 (Drc3) tagged with PA at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   dynein regulatory complex subunit 3 (Drc3 Mouse)
   

   Use

   Tags

   PA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-ATG101-ATG13 HORMA

   Plasmid

   #99332

   Purpose

   Mammalian expression of one subcomplex from the Human ULK1 complex

   Depositor

   James Hurley

   Article

   Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

   Inserts

   ATG101 (ATG101 Human)
   ATG13 (ATG13 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   p10 and pH

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1.Gαq-LgB97

   Plasmid

   #134360

   Purpose

   Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαq-LgB97 (GNAQ Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1.Gαi1-LgB91

   Plasmid

   #134342

   Purpose

   Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαi1-LgB91 (GNAI1 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_Thy1StTA

   Plasmid

   #97411

   Purpose

   TET driver for amplified expression in cortical neurons

   Depositor

   Tetsuo Yamamori

   Article

   Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse thy1PSs

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

   Plasmid

   #159817

   Purpose

   Positive control for germline GFP expression from simple array in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-sACt-mCherry-NLS

   Plasmid

   #138214

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   sACt-mCherry-NLS (Adcy10 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXW117As(RS-E11-RinA)2

   Plasmid

   #123146

   Purpose

   Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J117, ParsR, PhrpLE, Pecf11

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fireworks RFP[PTC+] (AVA2626)

   Plasmid

   #85444

   Purpose

   Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-sACt-mCherry-NES

    Plasmid

    #138215

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NES (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Fireworks GFP[PTC+] (AVA2600)

    Plasmid

    #85446

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pXW109Hg(RS-RinA-E11)2

    Plasmid

    #123151

    Purpose

    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT, PhrpLE, PrinA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRL838

    Plasmid

    #70680

    Purpose

    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

    Depositor

    Peter Wolk

    Article

    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Lyn-sACt-mCherry

    Plasmid

    #138216

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    Lyn-sACt-mCherry (Adcy10 Rat)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

    Plasmid

    #44597

    Depositor

    Susan Semple-Rowland

    Article

    Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

    Insert

    EF1a-GFP:2A:CHER(HA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human elongation factor -1a (pos 2528-3971)

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-MBP-NRBF2 (1-159)

    Plasmid

    #99331

    Purpose

    Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

    Depositor

    James Hurley

    Article

    Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

    Insert

    Nuclear receptor-binding factor 2 (NRBF2 Human)
    

    Use

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    AA1-159 are present

    Promoter

    T7

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. EC2_2_dCas9_VP64_sgRNA

    Plasmid

    #163707

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VP64+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p1.1-mCherry

    Plasmid

    #162772

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    red fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p1.2-GS-eGFP

    Plasmid

    #162771

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1.Gαi3-LgB91

    Plasmid

    #134359

    Purpose

    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi3-LgB91 (GNAI3 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge