We narrowed to 6,231 results for: nls

1. pLenti-FNLSHF1-P2A-Puro

   Plasmid

   #136901

   Purpose

   Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHF1
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   R661A,Q695A,Q926A

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1a-3NLS-Sox2TALE-GCN4

   Plasmid

   #120548

   Purpose

   Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

   Depositor

   Yu Wang

   Article

   Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

   Insert

   NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EF1a-3NLS-NGN2TALE-GCN4

   Plasmid

   #120547

   Purpose

   Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

   Depositor

   Yu Wang

   Article

   Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

   Insert

   NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCSD_FRB-TALETS3-3xFLAG-2xNLS

   Plasmid

   #107306

   Purpose

   Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   TALE_VEGFA-TS3
   

   Use

   CRISPR

   Tags

   3x Flag, 2xNLS and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPHR-EYFP-hGCN5-NLS

   Plasmid

   #103827

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-hGCN5-NLS (CRY2, KAT2A Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DsRed-CPI-17-T38A-NLS

   Plasmid

   #100792

   Purpose

   Mammalian expression of Fluorescent CPI-17 neutral mutation nuclear

   Depositor

   Joseph Gordon

   Insert

   protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
   

   Use

   Tags

   DsRed

   Expression

   Mammalian

   Mutation

   T38A

   Promoter

   CMV

   Available Since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DsRed-CPI-17-T38E-NLS

   Plasmid

   #100790

   Purpose

   Mammalian expression of Fluorescent CPI-17 phospho mimetic nuclear

   Depositor

   Joseph Gordon

   Insert

   CPI-17-T38E-NLS (PPP1R14A Human)
   

   Use

   Tags

   DsRed

   Expression

   Mammalian

   Mutation

   T38E

   Promoter

   CMV

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEN_TTmiRc2 3XFLAG-EGFP-NLS

   Plasmid

   #87778

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   eGFP
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

   Plasmid

   #87777

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK7-EE
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

    Plasmid

    #87776

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4 (MAP2K4 Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-myc-mCE NLS

    Plasmid

    #82465

    Purpose

    Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    deleted amino acids Nuclear Localization Signal

    Promoter

    CMV

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBABE-3xFLAG-NLS-TPP1-OB

    Plasmid

    #79060

    Purpose

    Retroviral expression of 3xFLAG tagged, nuclear localized wild-type TPP1-OB folding domain

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TPP1 (TPP1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Wild-type TPP-OB folding domain

    Promoter

    CMV

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pLPC-Flag-CHES1L-dC-NLS

    Plasmid

    #51840

    Purpose

    Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Flag and NLS

    Expression

    Mutation

    contains CHES1 amino acids 1-205

    Promoter

    Available Since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLPC-Flag-CHES1LdFC-NLS

    Plasmid

    #51838

    Purpose

    Flag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLS

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Flag and NLS

    Expression

    Mutation

    contains CHES1 amino acids 1-109

    Promoter

    Available Since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

    Plasmid

    #31484

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    EF1s HA NLS Sce(opt) T2A IFP1.4
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    "Opt" indicates the I-SceI coding seque…

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-fDIO-ArgiNLS-mKate2

    Plasmid

    #220607

    Purpose

    Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-fDIO-ArgiNLS-oScarlet

    Plasmid

    #220606

    Purpose

    Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    oScarlet
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

    Plasmid

    #196703

    Purpose

    Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 D210N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D210N, catalytically inactive

    Promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICH47811-pAtAct2::Cas9-nls::tSlATPase

    Plasmid

    #160221

    Purpose

    Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtACT2::Cas9-nls::tSlATPase
    

    Use

    Synthetic Biology; Moclo toolkit level 1 acceptor…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

    Plasmid

    #71396

    Purpose

    2Gi2R assay

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Inserts

    2xGFP:NES
    2xRFP:NLS
    

    Use

    Lentiviral

    Tags

    IRES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha and IRES

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge