We narrowed to 1,335 results for: piggyBac

1. OA-1067K

   Plasmid

   #164848

   Purpose

   Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

   Depositor

   Omar Akbari

   Article

   Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

   Insert

   4 gRNAs and 3xP3-tdTomato
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. JPF0419d

   Plasmid

   #124020

   Purpose

   Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187949

   Purpose

   SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   SMASh-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102892

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-RTP1S-Integration

   Plasmid

   #129458

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP1 (Rtp1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Removal of the first 36 amino acids of the protein

   Promoter

   TRE (Tet-On)

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. JPF0535a

   Plasmid

   #124042

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-TOX2

   Plasmid

   #192920

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of TOX2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   TOX2 (TOX2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-Haunt E1-PuroT2AmCherry-4A KI

   Plasmid

   #92164

   Purpose

   For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

   Depositor

   Xiaohua Shen

   Article

   Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

   Insert

   Haunt P-Inr2-cDNA KI homology arms
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLR5-CAG-NLS-tdiRFP670-2A-LBR

   Plasmid

   #82620

   Purpose

   Expresses NLS-tdiRFP-2A-LBR in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KA2880_pPB-TREtight-FLAG-Esrrb-InO

    Plasmid

    #124178

    Purpose

    piggyBac-based Dox-inducible expression of FLAG-Esrrb

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    FLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CAG-Haunt-EF1a-DsRed

    Plasmid

    #92162

    Purpose

    Haunt in trans overexpression plasmid

    Depositor

    Xiaohua Shen

    Article

    Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

    Insert

    Haunt (Halr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-BE3-3NLS

    Plasmid

    #119032

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JPF0419v

    Plasmid

    #124021

    Purpose

    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KA2944_pPB-TREtight-Esrrb-FLAG-InO

    Plasmid

    #124179

    Purpose

    piggyBac-based Dox-inducible expression of Esrrb-FLAG

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-CAG-HRKI(Haunt P-Inr2-cDNA KI)

    Plasmid

    #92163

    Purpose

    For lncRNA CAG promoter homologue recombination mediated knockin

    Depositor

    Xiaohua Shen

    Article

    Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

    Insert

    Haunt CAG KI homology arms
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

    Plasmid

    #242578

    Purpose

    PiggyBac transposon flanked, Tet-on, gateway destination vector.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    attR1-CmR-ccdB-attR2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TA-ERN-SETD5

    Plasmid

    #236616

    Purpose

    Expresses human SETD5 in mammalian cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

    Insert

    SETD5 (SETD5 Human)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-PE iSM

    Plasmid

    #235995

    Purpose

    Expresses iSM (iSpyMac) prime editor and pegRNA in mammalian cells

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    iSM_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    bpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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