We narrowed to 6,524 results for: poly

1. pCfb13211

   Plasmid

   #219886

   Purpose

   The base plasmid of TUNEYALI for TF21

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF21 (YALI1_E20635g) and homologous arm matching TF21
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfb13215

   Plasmid

   #219890

   Purpose

   The base plasmid of TUNEYALI for TF25

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF25 (YALI1_B18134g) and homologous arm matching TF25
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfb13221

   Plasmid

   #219896

   Purpose

   The base plasmid of TUNEYALI for TF31

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF31 (YALI1_D18727g) and homologous arm matching TF31
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfb13235

   Plasmid

   #219908

   Purpose

   The base plasmid of TUNEYALI for TF45

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF45 ( YALI1_B23373g) and homologous arm matching TF45
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfb13212

   Plasmid

   #219887

   Purpose

   The base plasmid of TUNEYALI for TF22

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF22 (YALI1_C25288g) and homologous arm matching TF22
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCfb13202

   Plasmid

   #219877

   Purpose

   The base plasmid of TUNEYALI forTF12

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF12 (YALI1_C06870g) and homologous arm matching TF12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCfb13234

   Plasmid

   #219907

   Purpose

   The base plasmid of TUNEYALI for TF44

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF44 (YALI1_B22561g) and homologous arm matching TF44
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfb13219

   Plasmid

   #219894

   Purpose

   The base plasmid of TUNEYALI for TF29

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF29 (YALI1_C09133g) and homologous arm matching TF29
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfb13248

   Plasmid

   #219919

   Purpose

   The base plasmid of TUNEYALI for TF58

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF58 (YALI1_F37742g) and homologous arm matching TF58
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAIP4_6WEY

    Plasmid

    #234234

    Purpose

    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAIP4_5HIH

    Plasmid

    #234205

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAIP4_2H2Z

    Plasmid

    #234147

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Use

    Tags

    Polyhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAIP4_5EF9

    Plasmid

    #234154

    Purpose

    Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5EF9 (PB2 Influenza B virus (B/Lee/1940))
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. JC Mad-1

    Plasmid

    #25626

    Depositor

    Peter Howley

    Article

    Howley et al J Virol. 1980 Dec . 36(3):878-82.

    Insert

    JC Virus Mad-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pDONR207-MpTubulin beta 3

    Plasmid

    #100594

    Purpose

    Gateway entry vector for MpTubulin beta 3

    Depositor

    Yutaka Kodama

    Article

    Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

    Insert

    MpTubulin beta 3
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOpen-BsupolLF

    Plasmid

    #165563

    Purpose

    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Bsu DNA Polymerase I, Large Fragment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAsylum

    Plasmid

    #24754

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-PfuX7

    Plasmid

    #182364

    Purpose

    Expresses PfuX7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

    Insert

    PfuX7 DNA polymerase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    V93Q

    Promoter

    T7

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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