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pAAV-CAG-miRFP680

Plasmid

#197203

Purpose

Expresses the protein of miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Use

AAV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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p5059

Plasmid

#198154

Purpose

bacterial expression of steC::HaloTag fusion

Depositor

Michael Hensel

Insert

PsteC steC::L16::HaloTag::HA

Tags

HaloTag-HA

Expression

Bacterial

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEY46

Plasmid

#191050

Purpose

glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-1 (glr-1 Nematode)

Expression

Worm

Mutation

None

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY38

Plasmid

#191042

Purpose

unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-8 (unc-8 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY15

Plasmid

#191021

Purpose

flp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-1 (flp-1 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY20

Plasmid

#191026

Purpose

gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-18 (gcy-18 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY48

Plasmid

#191052

Purpose

klp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

klp-6 (klp-6 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY47

Plasmid

#191051

Purpose

gcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-21 (gcy-21 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi Bla mAmetrine

Plasmid

#183257

Purpose

Stable expression of blue fluorescent mAmetrine protein in a Sleeping Beauty transposon vector.

Depositor

Alexander McLellan

Publication

He et al Int J Mol Sci. 2020 Dec 4;21(23). pii: i

Insert

mAmetrime

Expression

Mammalian

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pIT6EG7ml

Plasmid

#185886

Purpose

In vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (14 copy).

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

see comments

Expression

Yeast

Mutation

ERG20 F96W N127W

Available since

Sept. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ec2Avs2

Plasmid

#188790

Purpose

Avs2 from Escherichia coli NCTC10650

Depositor

Feng Zhang

Publication

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs2

Expression

Bacterial

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT1

Plasmid

#183282

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT1

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT3B

Plasmid

#183284

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT3B

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ESR1

Plasmid

#183288

Purpose

All-in-One CRISPRko system with a guide RNA that targets ESR1 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ESR1

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1B

Plasmid

#183338

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1B

Use

Lentiviral

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1A

Plasmid

#183337

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1A

Use

Lentiviral

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MYC

Plasmid

#183330

Purpose

All-in-One CRISPRko system with a guide RNA that targets MYC gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MYC

Use

Lentiviral

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2B

Plasmid

#183325

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2B

Use

Lentiviral

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PDGFRB

Plasmid

#183314

Purpose

All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PDGFRB

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_JAK2

Plasmid

#183303

Purpose

All-in-One CRISPRko system with a guide RNA that targets JAK2 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

JAK2

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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